• Immunohistochemistry analysis of SRPX in paraffin-embedded mouse kidney using SRPX Rabbit polyclonal antibody (STJ25698) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Mouse heart, using SRPX Rabbit polyclonal antibody (STJ25698) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SRPX antibody (40-320) (STJ25698)

SKU:
STJ25698

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Host: Rabbit
Applications: WB/IHC
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SRPX (40-320) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SRPX
Gene ID: 8406
Uniprot ID: SRPX_HUMAN
Immunogen Region: 40-320
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 40-320 of human SRPX (NP_006298.1).
Immunogen Sequence: EDDEVGYSHPRYKDTPWCSP IKVKYGDVYCRAPQGGYYKT ALGTRCDIRCQKGYELHGSS LLICQSNKRWSDKVICKQKR CPTLAMPANGGFKCVDGAYF NSRCEYYCSPGYTLKGERTV TCMDNKAWSGRPASCVDMEP PRIKCPSVKERIAEPNKLTV RVSWETPEGRDTADGILTDV ILKGLPPGSNFPEGDHKIQY TVYDRAENKGTCKFRVKVRV KRCGKLNAPENGYMKCSSD
Tissue Specificity Detected in fibroblasts (at protein level). Retina and heart.less in placenta, pancreas, lung, liver, skeletal muscle, kidney and brain.
Function May be involved in phagocytosis during disk shedding, cell adhesion to cells other than the pigment epithelium or signal transduction.
Protein Name Sushi Repeat-Containing Protein Srpx
Cellular Localisation Cell Surface
Possibly Surface Of Photoreceptor Cell
Alternative Antibody Names Anti-Sushi Repeat-Containing Protein Srpx antibody
Anti-SRPX antibody
Anti-ETX1 antibody

Information sourced from Uniprot.org

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