Anti-SREBF1 antibody [SRM14917] (STJ11109647)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthetic peptide. This information is considered to be commercially sensitive.
General Information
| Short Description | Rabbit monoclonal SREBF1 antibody for use in WB, IHC-P and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM14917 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity purification |
| Dilution Range | WB, 1:1000-1:10000IHC-P, 1:200-1:800ELISA, Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | Srebf1 |
| Gene ID | 20787 |
| Uniprot ID | SRBP1_MOUSE |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
Additional Info
| Tissue Specificity | Isoform SREBP-1C: Predominant isoform expressed in most tissues. Predominates in liver, adrenal gland, brain and adipose tissue. Also found in kidney, thymus, testis, muscle, jejunum, and ileum. Isoform SREBP-1A: Expressed only in select tissues, such as intestinal epithelial, heart, macrophage and bone marrow dendritic cells. Also found in kidney, thymus, testis, muscle, jejunum, and ileum. |
| Post Translational Modifications | Sterol regulatory element-binding protein 1: Processed in the Golgi apparatus, releasing the protein from the membrane. At low cholesterol the SCAP-SREBP complex is recruited into COPII vesicles for export from the endoplasmic reticulum. In the Golgi, complex SREBPs are cleaved sequentially by site-1 (MBTPS1, S1P) and site-2 (MBTPS2, S2P) proteases. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain, releasing the transcription factor from the Golgi membrane. Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression. Phosphorylation at Ser-389 by SIK1 represses activity possibly by inhibiting DNA-binding. Sterol regulatory element-binding protein 1: SCAP-free SREBF1 is ubiquitinated by the BCR(ARMC5) complex, leading to its degradation. Processed sterol regulatory element-binding protein 1: Ubiquitinated.the nuclear form has a rapid turnover and is rapidly ubiquitinated and degraded by the proteasome in the nucleus. |
| Function | Sterol regulatory element-binding protein 1: Precursor of the transcription factor form (Processed sterol regulatory element-binding protein 1), which is embedded in the endoplasmic reticulum membrane. Low sterol concentrations promote processing of this form, releasing the transcription factor form that translocates into the nucleus and activates transcription of genes involved in cholesterol biosynthesis and lipid homeostasis. Processed sterol regulatory element-binding protein 1: Key transcription factor that regulates expression of genes involved in cholesterol biosynthesis and lipid homeostasis. Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3'). Regulates the promoters of genes involved in cholesterol biosynthesis and the LDL receptor (LDLR) pathway of sterol regulation. Isoform SREBP-1A: Isoform expressed only in select tissues, which has higher transcriptional activity compared to SREBP-1C. Able to stimulate both lipogenic and cholesterogenic gene expression. Has a role in the nutritional regulation of fatty acids and triglycerides in lipogenic organs such as the liver. Required for innate immune response in macrophages by regulating lipid metabolism. Isoform SREBP-1C: Predominant isoform expressed in most tissues, which has weaker transcriptional activity compared to isoform SREBP-1A. Primarily controls expression of lipogenic gene. Strongly activates global lipid synthesis in rapidly growing cells. |
| Protein Name | Sterol Regulatory Element-Binding Protein 1Srebp-1Sterol Regulatory Element-Binding Transcription Factor 1 Cleaved Into - Processed Sterol Regulatory Element-Binding Protein 1Transcription Factor Srebf1 |
| Database Links | Reactome: R-MMU-1655829 Q9WTN3-1Reactome: -MMU-191273 Q9WTN3-1 |
| Cellular Localisation | Sterol Regulatory Element-Binding Protein 1: Endoplasmic Reticulum MembraneMulti-Pass Membrane ProteinGolgi Apparatus MembraneCytoplasmic VesicleCopii-Coated Vesicle MembraneAt High Sterol ConcentrationsThe Scap-Srebp Is Retained In The Endoplasmic ReticulumLow Sterol Concentrations Promote Recruitment Into Copii-Coated Vesicles And Transport Of The Scap-Srebp To The GolgiWhere It Is ProcessedProcessed Sterol Regulatory Element-Binding Protein 1: Nucleus |
| Alternative Antibody Names | Anti-Sterol Regulatory Element-Binding Protein 1 antibodyAnti-Srebp-1 antibodyAnti-Sterol Regulatory Element-Binding Transcription Factor 1 Cleaved Into - Processed Sterol Regulatory Element-Binding Protein 1 antibodyAnti-Transcription Factor Srebf1 antibodyAnti-Srebf1 antibodyAnti-Srebp1 antibody |
Information sourced from Uniprot.org