• Western blot analysis of lysates from Mouse skin, using SRD5A1 Rabbit polyclonal antibody (STJ116987) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SRD5A1 antibody (100-200) (STJ116987)

SKU:
STJ116987

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SRD5A1 (100-200) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SRD5A1
Gene ID: 6715
Uniprot ID: S5A1_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human SRD5A1 (NP_001038.1).
Immunogen Sequence: LIYPFLMRGGKPMPLLACTM AIMFCTCNGYLQSRYLSHCA VYADDWVTDPRFLIGFGLWL TGMLINIHSDHILRNLRKPG DTGYKIPRGGLFEYVTAANY F
Tissue Specificity Liver and prostate (at a low level).
Function Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.
Protein Name 3-Oxo-5-Alpha-Steroid 4-Dehydrogenase 1
Sr Type 1
Steroid 5-Alpha-Reductase 1
S5ar 1
Database Links Reactome: R-HSA-193048
Cellular Localisation Microsome Membrane
Multi-Pass Membrane Protein
Endoplasmic Reticulum Membrane
Alternative Antibody Names Anti-3-Oxo-5-Alpha-Steroid 4-Dehydrogenase 1 antibody
Anti-Sr Type 1 antibody
Anti-Steroid 5-Alpha-Reductase 1 antibody
Anti-S5ar 1 antibody
Anti-SRD5A1 antibody

Information sourced from Uniprot.org

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