| Host: |
Rabbit |
| Applications: |
IF |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-SQSTM1 is suitable for use in Immunofluorescence research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
S4923RM |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 0.05%BSA, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
Sqstm1 |
| Gene ID: |
18412 |
| Uniprot ID: |
SQSTM_MOUSE |
| Immunogen: |
A synthesized peptide derived from human SQSTM1. |
| Tissue Specificity | Widely expressed. |
| Post Translational Modifications | Phosphorylated. May be phosphorylated by PRKCZ. Phosphorylated in vitro by TTN. Phosphorylation at Ser-405 by ULK1 is stimulated by SESN2. Phosphorylated at Ser-405 by TBK1, leading to promote relocalization of 'Lys-63'-linked ubiquitinated STING1 to autophagosomes. Phosphorylation at Ser-351 by MTOR promotes interaction with KEAP1 and inactivation of the BCR(KEAP1) complex, promoting NFE2L2/NRF2 nuclear accumulation and expression of phase II detoxifying enzymes. Ubiquitinated by UBE2J1 and RNF26 at Lys-437: ubiquitinated SQSTM1 attracts specific vesicle-associated adapters, forming a molecular bridge that restrains cognate vesicles in the perinuclear region and organizes the endosomal pathway for efficient cargo transport. Deubiquitination by USP15 releases target vesicles for fast transport into the cell periphery. Ubiquitinated by the BCR(KEAP1) complex at Lys-422, increasing SQSTM1 sequestering activity and promoting its degradation. Ubiquitinated via 'Lys-29' and 'Lys-33'-linked polyubiquitination leading to xenophagic targeting of bacteria and inhibition of their replication. |
| Function | Autophagy receptor required for selective macroautophagy (aggrephagy). Functions as a bridge between polyubiquitinated cargo and autophagosomes. Interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family. Along with WDFY3, involved in the formation and autophagic degradation of cytoplasmic ubiquitin-containing inclusions (p62 bodies, ALIS/aggresome-like induced structures). Along with WDFY3, required to recruit ubiquitinated proteins to PML bodies in the nucleus. Also involved in autophagy of peroxisomes (pexophagy) in response to reactive oxygen species (ROS) by acting as a bridge between ubiquitinated PEX5 receptor and autophagosomes. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD. May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. Involved in endosome organization by retaining vesicles in the perinuclear cloud: following ubiquitination by RNF26, attracts specific vesicle-associated adapters, forming a molecular bridge that restrains cognate vesicles in the perinuclear region and organizes the endosomal pathway for efficient cargo transport. Promotes relocalization of 'Lys-63'-linked ubiquitinated STING1 to autophagosomes. Acts as an activator of the NFE2L2/NRF2 pathway via interaction with KEAP1: interaction inactivates the BCR(KEAP1) complex, promoting nuclear accumulation of NFE2L2/NRF2 and subsequent expression of cytoprotective genes. Sequesters tensin TNS2 into cytoplasmic puncta, promoting TNS2 ubiquitination and proteasomal degradation. |
| Protein Name | Sequestosome-1Stone14Ubiquitin-Binding Protein P62 |
| Database Links | Reactome: R-MMU-205043Reactome: -MMU-209543Reactome: -MMU-209560Reactome: -MMU-5205685Reactome: -MMU-9020702Reactome: -MMU-9664873Reactome: -MMU-9755511 |
| Cellular Localisation | CytoplasmCytosolPreautophagosomal StructureLate EndosomeNucleusEndoplasmic ReticulumLysosomeCytoplasmic VesicleAutophagosomePml BodyMyofibrilSarcomereIn Cardiac MusclesLocalizes To The Sarcomeric BandMay Also Localize To The Hepatocellular CarcinomaColocalizes With Trim13 In The Perinuclear Endoplasmic ReticulumCommonly Found In Inclusion Bodies Containing Polyubiquitinated Protein AggregatesCo-Localizes With Trim5 In The Cytoplasmic BodiesWhen Nuclear Export Is Blocked By Treatment With Leptomycin BAccumulates In Pml Bodies |
| Alternative Antibody Names | Anti-Sequestosome-1 antibodyAnti-Stone14 antibodyAnti-Ubiquitin-Binding Protein P62 antibodyAnti-Sqstm1 antibodyAnti-A170 antibodyAnti-STAP antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
