| Post Translational Modifications | Methylated by PRMT1/HRMT1L2 and PRMT5/SKB1. Methylation negatively regulates interaction with P-TEFb and RNA polymerase II. Phosphorylated by CDK7 and CDK9. Phosphorylation by P-TEFb alleviates transcriptional pausing. Phosphorylation may also stimulate interaction with PIN1. Bulk phosphorylation occurs predominantly in mitosis. Ubiquitinated by UBR5 when not assembled in the DSIF complex, leading to its degradation: UBR5 recognizes and binds a degron that is not accessible when SUPT5H is part of the DSIF complex. |
| Function | Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. |
| Protein Name | Transcription Elongation Factor Spt5Drb Sensitivity-Inducing Factor Large SubunitDsif Large Subunit |
| Database Links | Reactome: R-MMU-112382Reactome: -MMU-113418Reactome: -MMU-674695Reactome: -MMU-6796648Reactome: -MMU-6807505Reactome: -MMU-72086Reactome: -MMU-75955Reactome: -MMU-77075 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Transcription Elongation Factor Spt5 antibodyAnti-Drb Sensitivity-Inducing Factor Large Subunit antibodyAnti-Dsif Large Subunit antibodyAnti-Supt5h antibodyAnti-Supt5 antibody |
Information sourced from Uniprot.org
