Western blot analysis of lysates from mouse brain, using SPRED1 Rabbit polyclonal antibody (STJ117169) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SPRED1 antibody (120-320) (STJ117169)

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STJ117169
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SPRED1
Gene ID: 161742
Uniprot ID: SPRE1_HUMAN
Immunogen Region: 120-320
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 120-320 of human SPRED1 (NP_689807.1).
Immunogen Sequence: IEDISQGCPESKNEAEGADD LQANEEDSSSSLVKDHLFQQ ETVVTSEPYRSSNIRPSPFE DLNARRVYMQSQANQITFGQ PGLDIQSRSMEYVQRQISKE CGSLKSQNRVPLKSIRHVSF QDEDEIVRINPRDILIRRYA DYRHPDMWKNDLERDDADSS IQFSKPDSKKSDYLYSCGDE TKLSSPKDSVVFKTQPSSLK I
Tissue Specificity Weakly expressed in embryonic cell line HEK293.
Post Translational Modifications Palmitoylated by ZDHHC17/HIP14. Phosphorylated on tyrosine. Ubiquitinated.
Function Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase. Negatively regulates hematopoiesis of bone marrow. Inhibits fibroblast growth factor (FGF)-induced retinal lens fiber differentiation, probably by inhibiting FGF-mediated phosphorylation of ERK1/2. Attenuates actin stress fiber formation via inhibition of TESK1-mediated phosphorylation of cofilin. Inhibits TGFB-induced epithelial-to-mesenchymal transition in lens epithelial cells.
Protein Name Sprouty-Related - Evh1 Domain-Containing Protein 1
Spred-1
Hspred1
Database Links Reactome: R-HSA-5658442
Reactome: R-HSA-5658623
Reactome: R-HSA-6802953
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Membrane
Caveola
Nucleus
Localized In Cholesterol-Rich Membrane Raft/Caveola Fractions
Alternative Antibody Names Anti-Sprouty-Related - Evh1 Domain-Containing Protein 1 antibody
Anti-Spred-1 antibody
Anti-Hspred1 antibody
Anti-SPRED1 antibody

Information sourced from Uniprot.org

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