• Western blot analysis of various lysates, using Osteopontin antibody (STJ25679) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-SPP1 antibody (17-314) (STJ25679)

SKU:
STJ25679

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SPP1 (17-314) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SPP1
Gene ID: 6696
Uniprot ID: OSTP_HUMAN
Immunogen Region: 17-314
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 17-314 of human Osteopontin (NP_001035147.1).
Immunogen Sequence: IPVKQADSGSSEEKQLYNKY PDAVATWLNPDPSQKQNLLA PQNAVSSEETNDFKQETLPS KSNESHDHMDDMDDEDDDDH VDSQDSIDSNDSDDVDDTDD SHQSDESHHSDESDELVTDF PTDLPATEVFTPVVPTVDTY DGRGDSVVYGLRSKSKKFRR PDIQYPDATDEDITSHMESE ELNGAYKAIPVAQDLNAPSD WDSRGKDSYETSQLDDQSAE THSHKQSRLYKRKANDESN
Tissue Specificity Detected in cerebrospinal fluid and urine (at protein level). Bone. Found in plasma.
Post Translational Modifications Forms covalent cross-links mediated by transglutaminase TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers, increasing its collagen binding properties. Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif. The phosphorylated form inhibits hydroxyapatite crystallization. Dephosphorylation via a mechanism involving ALPL/TNAP promotes hydroxyapatite crystallization. O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62.
Function Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
Protein Name Osteopontin
Bone Sialoprotein 1
Nephropontin
Secreted Phosphoprotein 1
Spp-1
Urinary Stone Protein
Uropontin
Database Links Reactome: R-HSA-1474228
Reactome: R-HSA-186797
Reactome: R-HSA-216083
Reactome: R-HSA-381426
Reactome: R-HSA-8949275
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Alternative Antibody Names Anti-Osteopontin antibody
Anti-Bone Sialoprotein 1 antibody
Anti-Nephropontin antibody
Anti-Secreted Phosphoprotein 1 antibody
Anti-Spp-1 antibody
Anti-Urinary Stone Protein antibody
Anti-Uropontin antibody
Anti-SPP1 antibody
Anti-BNSP antibody
Anti-OPN antibody
Anti-PSEC0156 antibody

Information sourced from Uniprot.org

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