• Formalin-fixed, paraffin-embedded human lymph node stained with anti-CD43 antibody using peroxidase-conjugate and DAB chromogen. Note the cell membrane staining of perifollicular T-cells
  • Formalin-fixed, paraffin-embedded human lymph node stained with anti-CD43 antibody using peroxidase-conjugate and DAB chromogen. Note the cell membrane staining of perifollicular T-cells

Anti-SPN antibody (STJ180058)

SKU:
STJ180058
Current Stock:
Host: Mouse
Applications: IHC-p
Reactivity: Human
Short Description: Mouse monoclonal primary antibody against SPN.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Monoclonal
Clone ID: DF‐T1
Conjugation: Unconjugated
Formulation: Purified antibody diluted in Tris‐HCI buffer containing stabilizing protein and <0.1% sodium azide.
Storage Instruction: Store at 2‐8°C for up to 24 months. Predilute: Ready to use, no reconstitution necessary. Concentrate: Use dilution range and appropriate lab‐standardized diluent. Stability after dilution: 7 days at 24°C, 3 months at 2‐8°C, 6months at ‐20°C.
Isotype: IgG1 Kappa
Dilution Range: 1:100
Uniprot ID: LEUK_HUMAN
Gene ID: 6693
Gene Symbol: SPN
Immunogen: Stimulated human leukocytes
Background CD43 (leukosialin, sialophorin, or leukocyte sialoglycoprotein) is a cell surface glycoprotein which is expressed on all thymocytes and T‐cells. CD43 is involved in activation of T cells, B cells, NK cells, and monocytes.
Tissue Specificity Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.
Post Translational Modifications Glycosylated.has a high content of sialic acid and O-linked carbohydrate structures. Phosphorylation at Ser-355 is regulated by chemokines, requires its association with ERM proteins (EZR, RDX and MSN) and is essential for its function in the regulation of T-cell trafficking to lymph nodes. Has a high content of sialic acid and O-linked carbohydrate structures. Cleavage by CTSG releases its extracellular domain and triggers its intramembrane proteolysis by gamma-secretase releasing the CD43 cytoplasmic tail chain (CD43-ct) which translocates to the nucleus. CD43 cytoplasmic tail: Sumoylated.
Function Predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. Positively regulates T-cell trafficking to lymph-nodes via its association with ERM proteins (EZR, RDX and MSN). Negatively regulates Th2 cell differentiation and predisposes the differentiation of T-cells towards a Th1 lineage commitment. Promotes the expression of IFN-gamma by T-cells during T-cell receptor (TCR) activation of naive cells and induces the expression of IFN-gamma by CD4(+) T-cells and to a lesser extent by CD8(+) T-cells. Plays a role in preparing T-cells for cytokine sensing and differentiation into effector cells by inducing the expression of cytokine receptors IFNGR and IL4R, promoting IFNGR and IL4R signaling and by mediating the clustering of IFNGR with TCR. Acts as a major E-selectin ligand responsible for Th17 cell rolling on activated vasculature and recruitment during inflammation. Mediates Th17 cells, but not Th1 cells, adhesion to E-selectin. Acts as a T-cell counter-receptor for SIGLEC1. CD43 cytoplasmic tail: Protects cells from apoptotic signals, promoting cell survival.
Database Links Reactome: R-HSA-202733
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Cell Projection
Microvillus
Uropodium
Localizes To The Uropodium And Microvilli Via Its Interaction With Erm Proteins (Ezr
Rdx And Msn)
Cd43 Cytoplasmic Tail: Nucleus
Nucleus
Pml Body
Protein Name Leukosialin
Gpl115
Galactoglycoprotein
Galgp
Leukocyte Sialoglycoprotein
Sialophorin
Cd Antigen Cd43 Cleaved Into - Cd43 Cytoplasmic Tail
Cd43-Ct
Cd43ct
Alternative Names Anti-Leukosialin antibody
Anti-Gpl115 antibody
Anti-Galactoglycoprotein antibody
Anti-Galgp antibody
Anti-Leukocyte Sialoglycoprotein antibody
Anti-Sialophorin antibody
Anti-Cd Antigen Cd43 Cleaved Into - Cd43 Cytoplasmic Tail antibody
Anti-Cd43-Ct antibody
Anti-Cd43ct antibody
Anti-SPN antibody
Anti-CD43 antibody
Positive Control Tonsil, CLL/SLL, or lymph node.

Information sourced from Uniprot.org

6 months standard