| Host: | Mouse |
| Applications: | IHC-P |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-SPN is suitable for use in Immunohistochemistry research applications. |
| Clonality : | Monoclonal |
| Clone ID : | DF‐T1 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1/Kappa |
| Formulation: | Tris-HCI buffer containing stabilizing protein (BSA) and <0.1% ProClin |
| Purification: | Affinity purified |
| Dilution Range: | 1:100 |
| Storage Instruction: | Store at 2‐8°C for up to 24 months. Predilute: Ready to use, no reconstitution necessary. Concentrate: Use dilution range and appropriate lab‐standardized diluent. Stability after dilution: 7 days at 24°C, 3 months at 2‐8°C, 6months at ‐20°C. |
| Gene Symbol: | SPN |
| Gene ID: | 6693 |
| Uniprot ID: | LEUK_HUMAN |
| Specificity: | Positive control: Tonsil, CLL/SLL, or lymph node |
| Immunogen: | Stimulated human leukocytes |
| Tissue Specificity | Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas. |
| Post Translational Modifications | Glycosylated.has a high content of sialic acid and O-linked carbohydrate structures. Phosphorylation at Ser-355 is regulated by chemokines, requires its association with ERM proteins (EZR, RDX and MSN) and is essential for its function in the regulation of T-cell trafficking to lymph nodes. Has a high content of sialic acid and O-linked carbohydrate structures. Cleavage by CTSG releases its extracellular domain and triggers its intramembrane proteolysis by gamma-secretase releasing the CD43 cytoplasmic tail chain (CD43-ct) which translocates to the nucleus. CD43 cytoplasmic tail: Sumoylated. |
| Function | Predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. Positively regulates T-cell trafficking to lymph-nodes via its association with ERM proteins (EZR, RDX and MSN). Negatively regulates Th2 cell differentiation and predisposes the differentiation of T-cells towards a Th1 lineage commitment. Promotes the expression of IFN-gamma by T-cells during T-cell receptor (TCR) activation of naive cells and induces the expression of IFN-gamma by CD4(+) T-cells and to a lesser extent by CD8(+) T-cells. Plays a role in preparing T-cells for cytokine sensing and differentiation into effector cells by inducing the expression of cytokine receptors IFNGR and IL4R, promoting IFNGR and IL4R signaling and by mediating the clustering of IFNGR with TCR. Acts as a major E-selectin ligand responsible for Th17 cell rolling on activated vasculature and recruitment during inflammation. Mediates Th17 cells, but not Th1 cells, adhesion to E-selectin. Acts as a T-cell counter-receptor for SIGLEC1. CD43 cytoplasmic tail: Protects cells from apoptotic signals, promoting cell survival. |
| Protein Name | Leukosialin Gpl115 Galactoglycoprotein Galgp Leukocyte Sialoglycoprotein Sialophorin Cd Antigen Cd43 Cleaved Into - Cd43 Cytoplasmic Tail Cd43-Ct Cd43ct |
| Database Links | Reactome: R-HSA-202733 Reactome: R-HSA-210991 |
| Cellular Localisation | Membrane Single-Pass Type I Membrane Protein Cell Projection Microvillus Uropodium Localizes To The Uropodium And Microvilli Via Its Interaction With Erm Proteins (Ezr Rdx And Msn) Cd43 Cytoplasmic Tail: Nucleus Nucleus Pml Body The Sumoylated Form Localizes To The Pml Body |
| Alternative Antibody Names | Anti-Leukosialin antibody Anti-Gpl115 antibody Anti-Galactoglycoprotein antibody Anti-Galgp antibody Anti-Leukocyte Sialoglycoprotein antibody Anti-Sialophorin antibody Anti-Cd Antigen Cd43 Cleaved Into - Cd43 Cytoplasmic Tail antibody Anti-Cd43-Ct antibody Anti-Cd43ct antibody Anti-SPN antibody Anti-CD43 antibody |
Information sourced from Uniprot.org