Anti-Splicing factor 3B subunit 4 antibody (Full-Length) [3A1] (STJA0040764)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2b
ImmunogenFull length recombinant human SF3B4 which was expressed in and purified from E. coli.
General Information
| Short Description | Mouse monoclonal anti-Splicing factor 3B subunit 4 (Full-Length) for use in FC, ICC and WB in Bovine, Human, Mouse, Other Mammals, Pig and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | FC/ICC/WB |
| Host | Mouse |
| Reactivity | Bovine/Human/Mouse/Other Mammals/Pig/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 3A1 |
| Isotype | IgG2b |
| Conjugation | Unconjugated |
| Purification | Affinity-purified |
| Dilution Range | WB 0.73611111111111116ICC 1:500-1:2000 |
| Formulation | Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide |
| Storage Instruction | Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. Aliquot and store at-20°C for a higher stability and at 2-8°C with an approp |
Target Information
| Gene Symbol | SF3B4 |
| Gene ID | 10262 |
| Uniprot ID | SF3B4_HUMAN |
| Immunogen | Full length recombinant human SF3B4 which was expressed in and purified from E. coli. |
| Immunogen Region | Full-Length |
| Immunogen Sequence | Human |
| Specificity | Human SF3B4 ; bovine; Porcine; mouse; rat; expected to react with other species due to sequence homology. |
Additional Info
| Function | Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, SF3B4 is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA. Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Also acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs. |
| Protein Name | Splicing Factor 3b Subunit 4Pre-Mrna-Splicing Factor Sf3b 49 Kda SubunitSpliceosome-Associated Protein 49Sap 49 |
| Database Links | Reactome: R-HSA-72163Reactome: R-HSA-72165 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Splicing Factor 3b Subunit 4 antibodyAnti-Pre-Mrna-Splicing Factor Sf3b 49 Kda Subunit antibodyAnti-Spliceosome-Associated Protein 49 antibodyAnti-Sap 49 antibodyAnti-SF3B4 antibodyAnti-SAP49 antibody |
Information sourced from Uniprot.org