• Immunofluorescence analysis of HeLa cells using SPINT2 antibody (STJ28832). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of MCF-7 cells, using SPINT2 Antibody (STJ28832) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SPINT2 antibody (28-197) (STJ28832)

SKU:
STJ28832

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SPINT2 (28-197) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SPINT2
Gene ID: 10653
Uniprot ID: SPIT2_HUMAN
Immunogen Region: 28-197
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 28-197 of human SPINT2 (NP_066925.1).
Immunogen Sequence: ADRERSIHDFCLVSKVVGRC RASMPRWWYNVTDGSCQLFV YGGCDGNSNNYLTKEECLKK CATVTENATGDLATSRNAAD SSVPSAPRRQDSEDHSSDMF NYEEYCTANAVTGPCRASFP RWYFDVERNSCNNFIYGGCR GNKNSYRSEEACMLRCFRQQ ENPPLPLGSK
Tissue Specificity Expressed in placenta, kidney, pancreas, prostate, testis, thymus, and trachea.
Function Inhibitor of HGFAC. Also inhibits plasmin, and plasma and tissue kallikrein. Inhibits serine protease activity of TMPRSS13. Inhibits serine protease activity of ST14/matriptase in vitro.
Protein Name Kunitz-Type Protease Inhibitor 2
Hepatocyte Growth Factor Activator Inhibitor Type 2
Hai-2
Placental Bikunin
Database Links Reactome: R-HSA-6806942
Reactome: R-HSA-8852405
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Cytoplasm
Alternative Antibody Names Anti-Kunitz-Type Protease Inhibitor 2 antibody
Anti-Hepatocyte Growth Factor Activator Inhibitor Type 2 antibody
Anti-Hai-2 antibody
Anti-Placental Bikunin antibody
Anti-SPINT2 antibody
Anti-HAI2 antibody
Anti-KOP antibody

Information sourced from Uniprot.org

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