• Immunohistochemistry analysis of paraffin-embedded mouse brain using SPIN1 antibody (STJ28416) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using SPIN1 antibody (STJ28416) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using SPIN1 antibody (STJ28416) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SPIN1 antibody (1-262) (STJ28416)

SKU:
STJ28416

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SPIN1 (1-262) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SPIN1
Gene ID: 10927
Uniprot ID: SPIN1_HUMAN
Immunogen Region: 1-262
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-262 of human SPIN1 (NP_006708.2).
Immunogen Sequence: MKTPFGKTPGQRSRADAGHA GVSANMMKKRTSHKKHRSSV GPSKPVSQPRRNIVGCRIQH GWKEGNGPVTQWKGTVLDQV PVNPSLYLIKYDGFDCVYGL ELNKDERVSALEVLPDRVAT SRISDAHLADTMIGKAVEHM FETEDGSKDEWRGMVLARAP VMNTWFYITYEKDPVLYMYQ LLDDYKEGDLRIMPDSNDSP PAEREPGEVVDSLVGKQVEY AKEDGSKRTGMVIHQVEAK
Tissue Specificity Highly expressed in ovarian cancer tissues.
Post Translational Modifications Phosphorylated during oocyte meiotic maturation.
Function Chromatin reader that specifically recognizes and binds histone H3 both trimethylated at 'Lys-4' and asymmetrically dimethylated at 'Arg-8' (H3K4me3 and H3R8me2a) and acts as an activator of Wnt signaling pathway downstream of PRMT2. In case of cancer, promotes cell cancer proliferation via activation of the Wnt signaling pathway. Overexpression induces metaphase arrest and chromosomal instability. Localizes to active rDNA loci and promotes the expression of rRNA genes. May play a role in cell-cycle regulation during the transition from gamete to embryo. Involved in oocyte meiotic resumption, a process that takes place before ovulation to resume meiosis of oocytes blocked in prophase I: may act by regulating maternal transcripts to control meiotic resumption.
Protein Name Spindlin-1
Ovarian Cancer-Related Protein
Spindlin1
Cellular Localisation Nucleus
Nucleolus
Alternative Antibody Names Anti-Spindlin-1 antibody
Anti-Ovarian Cancer-Related Protein antibody
Anti-Spindlin1 antibody
Anti-SPIN1 antibody
Anti-OCR antibody
Anti-SPIN antibody

Information sourced from Uniprot.org

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