Anti-Spectrin, alpha II antibody (676-2, 400) (STJA0040968)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antibody was raised against a mix of five recombinant constructs containing the entire C-terminal region of human alpha-II spectrin (amino acids 676-2, 400).
General Information
| Short Description | Rabbit polyclonal anti-Spectrin, alpha II (676-2, 400) for use in ICC and WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ICC/WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Whole serum |
| Dilution Range | WB 1:5000-1:10000ICC 1:500-1:1000 |
| Formulation | Lyophilized with sodium azide. |
| Storage Instruction | Spin vial briefly before opening. Reconstitute with 100 ยตL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Store lyophilized, unopened vial at 2-8ยฐC or lower. After reconstitution, prepare aliquots and store at-20ยฐC to |
Target Information
| Gene Symbol | SPTAN1 |
| Gene ID | 6709 |
| Uniprot ID | SPTN1_HUMAN |
| Immunogen | The antibody was raised against a mix of five recombinant constructs containing the entire C-terminal region of human alpha-II spectrin (amino acids 676-2, 400). |
| Immunogen Region | 676-2, 400 |
| Immunogen Sequence | Human |
| Specificity | The antibody reacts with a 240 kDa band by Western blot on mouse sciatic nerve extract. Minor bands below may be seen and this likely represents in vivo proteolytic fragments of alpha-II spectrin. It has also been used successfully for immunocytochem |
Additional Info
| Post Translational Modifications | Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro. |
| Function | Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane. |
| Protein Name | Spectrin Alpha Chain - Non-Erythrocytic 1Alpha-Ii SpectrinFodrin Alpha ChainSpectrin - Non-Erythroid Alpha Subunit |
| Database Links | Reactome: R-HSA-264870Reactome: R-HSA-373753Reactome: R-HSA-375165Reactome: R-HSA-445095Reactome: R-HSA-5673001Reactome: R-HSA-6798695Reactome: R-HSA-6807878Reactome: R-HSA-9013420Reactome: R-HSA-9013424Reactome: R-HSA-9662360Reactome: R-HSA-9662361 |
| Cellular Localisation | CytoplasmCytoskeletonCell CortexExpressed Along The Cell Membrane In Podocytes And Presumptive Tubule Cells During Glomerulogenesis And Is Expressed Along Lateral Cell Margins In Tubule Cells |
| Alternative Antibody Names | Anti-Spectrin Alpha Chain - Non-Erythrocytic 1 antibodyAnti-Alpha-Ii Spectrin antibodyAnti-Fodrin Alpha Chain antibodyAnti-Spectrin - Non-Erythroid Alpha Subunit antibodyAnti-SPTAN1 antibodyAnti-NEAS antibodyAnti-SPTA2 antibody |
Information sourced from Uniprot.org