Anti-Spectrin alpha chain, non-erythrocytic 1 antibody (676-1043) [3D7] (STJA0040763)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenThis antibody was raised against a recombinant construct containing the seventh, eight and ninth repeats (amino acids 676-1043) of human alpha-II Spectrin. The 9th spectrin repeat also includes a Src-homology 3 domain. This construct was expressed in
General Information
| Short Description | Mouse monoclonal anti-Spectrin alpha chain, non-erythrocytic 1 (676-1043) for use in FC, ICC, IHC-F and WB in Bovine, Human, Mouse, Pig and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | FC/ICC/IHC-F/WB |
| Host | Mouse |
| Reactivity | Bovine/Human/Mouse/Pig/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 3D7 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Purification | Affinity-purified |
| Dilution Range | WB 1:5000-1:10000IHC 1:1000-1:2000ICC 1:1000-1:2000 |
| Formulation | Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide |
| Storage Instruction | Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. Aliquot and store at-20°C for a higher stability and at 2-8°C with an approp |
Target Information
| Gene Symbol | SPTAN1 |
| Gene ID | 6709 |
| Uniprot ID | SPTN1_HUMAN |
| Immunogen | This antibody was raised against a recombinant construct containing the seventh, eight and ninth repeats (amino acids 676-1043) of human alpha-II Spectrin. The 9th spectrin repeat also includes a Src-homology 3 domain. This construct was expressed in |
| Immunogen Region | 676-1043 |
| Immunogen Sequence | Human |
| Specificity | Human; bovine; porcine; rat;. |
Additional Info
| Post Translational Modifications | Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro. |
| Function | Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane. |
| Protein Name | Spectrin Alpha Chain - Non-Erythrocytic 1Alpha-Ii SpectrinFodrin Alpha ChainSpectrin - Non-Erythroid Alpha Subunit |
| Database Links | Reactome: R-HSA-264870Reactome: R-HSA-373753Reactome: R-HSA-375165Reactome: R-HSA-445095Reactome: R-HSA-5673001Reactome: R-HSA-6798695Reactome: R-HSA-6807878Reactome: R-HSA-9013420Reactome: R-HSA-9013424Reactome: R-HSA-9662360Reactome: R-HSA-9662361 |
| Cellular Localisation | CytoplasmCytoskeletonCell CortexExpressed Along The Cell Membrane In Podocytes And Presumptive Tubule Cells During Glomerulogenesis And Is Expressed Along Lateral Cell Margins In Tubule Cells |
| Alternative Antibody Names | Anti-Spectrin Alpha Chain - Non-Erythrocytic 1 antibodyAnti-Alpha-Ii Spectrin antibodyAnti-Fodrin Alpha Chain antibodyAnti-Spectrin - Non-Erythroid Alpha Subunit antibodyAnti-SPTAN1 antibodyAnti-NEAS antibodyAnti-SPTA2 antibody |
Information sourced from Uniprot.org