• Western blot analysis of extracts of various cell lines, using SPC25 antibody (STJ11101163) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-SPC25 antibody (120-224) (STJ11101163)

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STJ11101163

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SPC25 (120-224) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SPC25
Gene ID: 57405
Uniprot ID: SPC25_HUMAN
Immunogen Region: 120-224
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 120-224 of human SPC25 (NP_065726.1).
Immunogen Sequence: ANKANAERLKRLQKSADLYK DRLGLEIRKIYGEKLQFIFT NIDPKNPESPFMFSLHLNEA RDYEVSDSAPHLEGLAEFQE NVRKTNNFSAFLANVRKAFT ATVYN
Function Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules.
Protein Name Kinetochore Protein Spc25
Hspc25
Database Links Reactome: R-HSA-141444
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-5663220
Reactome: R-HSA-68877
Reactome: R-HSA-9648025
Cellular Localisation Nucleus
Chromosome
Centromere
Kinetochore
Localizes To Kinetochores From Late Prophase To Anaphase
Localizes Specifically To The Outer Plate Of The Kinetochore
Alternative Antibody Names Anti-Kinetochore Protein Spc25 antibody
Anti-Hspc25 antibody
Anti-SPC25 antibody
Anti-SPBC25 antibody
Anti-AD024 antibody

Information sourced from Uniprot.org

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