Anti-SPC24 antibody (1-197) (STJ119028)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal SPC24 (1-197) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:5000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SPC24 |
| Gene ID | 147841 |
| Uniprot ID | SPC24_HUMAN |
| Immunogen Region | 1-197 |
| Immunogen Sequence | MAAFRDIEEVSQGLLSLLGA NRAEAQQRRLLGRHEQVVER LLETQDGAEKQLREILTMEK EVAQSLLNAKEQVHQGGVEL QQLEAGLQEAGEEDTRLKAS LLQLTRELEELKEIEADLER QEKEVDEDTTVTIPSAVYVA QLYHQVSKIEWDYECEPGMV KGIHHGPSVAQPIHLDSTQL SRKFISDYLWSLVDTEW |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-197 of human SPC24 (NP_872319.1). |
Additional Info
| Function | Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules. |
| Protein Name | Kinetochore Protein Spc24Hspc24 |
| Database Links | Reactome: R-HSA-141444Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-5663220Reactome: R-HSA-68877Reactome: R-HSA-9648025 |
| Cellular Localisation | NucleusChromosomeCentromereKinetochoreLocalizes To Kinetochores From Late Prophase To AnaphaseLocalizes Specifically To The Outer Plate Of The Kinetochore |
| Alternative Antibody Names | Anti-Kinetochore Protein Spc24 antibodyAnti-Hspc24 antibodyAnti-SPC24 antibodyAnti-SPBC24 antibody |
Information sourced from Uniprot.org