• Western blot analysis of extracts of various cell lines, using SPATA4 antibody (STJ29745) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SPATA4 antibody (1-250) (STJ29745)

SKU:
STJ29745

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SPATA4 (1-250) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SPATA4
Gene ID: 132851
Uniprot ID: SPAT4_HUMAN
Immunogen Region: 1-250
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human SPATA4 (NP_653245.2).
Immunogen Sequence: MAAAGQEKGYLTQTAAALDK SPSLSPQLAAPIRGRPKKCL VYPHAPKSSRLSRSVLRWLQ GLDLSFFPRNINRDFSNGFL IAEIFCIYYPWELELSSFEN GTSLKVKLDNWAQLEKFLAR KKFKLPKELIHGTIHCKAGV PEILIEEVYTLLTHREIKSI QDDFVNFTDYSYQMRLPLVS RSTVSKSIKDNIRLSELLSN PNMLTNELKAEFLILLHMLQ RKLGRKLNPEWFDVKPTVG
Tissue Specificity Highly expressed in testis, the expression is observed precisely in seminiferous tubules.
Function May play a role in apoptosis regulation.
Protein Name Spermatogenesis-Associated Protein 4
Testis And Spermatogenesis Cell-Related Protein 2
Testis Spermatocyte Apoptosis-Related Gene 2 Protein
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Spermatogenesis-Associated Protein 4 antibody
Anti-Testis And Spermatogenesis Cell-Related Protein 2 antibody
Anti-Testis Spermatocyte Apoptosis-Related Gene 2 Protein antibody
Anti-SPATA4 antibody
Anti-TSARG2 antibody

Information sourced from Uniprot.org

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