Anti-SPAG8 antibody (220-420) (STJ113600)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal SPAG8 (220-420) antibody for use in WB and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SPAG8 |
| Gene ID | 26206 |
| Uniprot ID | SPAG8_HUMAN |
| Immunogen Region | 220-420 |
| Immunogen Sequence | LVADRVPNYTSWSQHCPWEP QKQPPWEFLQVLEPGARGLW KPPDIKGKLMVCYETLPRGQ CLLYNWEEERATNHLDQVPS MQDGSESFFFRHGHRGLLTM QLKSPMPSSTTQKDSYQPPG NVYWPLRGKREAMLEMLLQH QICKEVQAEQEPTRKLFEVE SVTHHDYRMELAQAGTPAPT KPHDYRQEQPETFWIQRAPQ L |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 220-420 of human SPAG8 (NP_758516.1). |
Additional Info
| Tissue Specificity | Expressed in testis (germ cells), but not in liver, kidney, prostate and small intestine. Expressed in airway epithelial cells. |
| Function | Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia axoneme, which is required for motile cilia beating. Plays a role in spermatogenesis by enhancing the binding of CREM isoform tau to its coactivator FHL5 and increasing the FHL5-regulated transcriptional activation of CREM isoform tau. Involved in the acrosome reaction and in binding of sperm to the zona pellucida. Plays a role in regulation of the cell cycle by controlling progression through the G2/M phase, possibly by delaying the activation of CDK1 which is required for entry into mitosis. May play a role in fertility and microtubule formation through interaction with RANBP9. |
| Protein Name | Sperm-Associated Antigen 8Hsd-1Sperm Membrane Protein 1Smp-1Sperm Membrane Protein Bs-84 |
| Database Links | |
| Cellular Localisation | CytoplasmNucleusCytoplasmic VesicleSecretory VesicleAcrosomeCytoskeletonMicrotubule Organizing CenterSpindleCilium AxonemeFlagellum AxonemeIn Mature Sperm CellsDetected In The Acrosomal Region Of The Head And In The Middle Piece Of The TailLocalized To The Nucleus And Cytoplasm Of Spermatocytes And Round Spermatids WhileIn Elongating SpermatidsExpressed In The Cytoplasm But Not In The NucleusDuring The Cell CycleLocalized On The Microtubule-Organizing Center (Mtoc) During ProphaseIn MetaphaseExtends Along Spindle MicrotubulesIn AnaphaseDetected On The Astral Microtubules And Mid-ZoneIn TelophaseRemains At The Mid-ZoneAfter CytokinesisReturns To The MtocMicrotubule Inner Protein (Mip) Part Of The Dynein-Decorated Doublet Microtubules (Dmts) In Cilia Axoneme |
| Alternative Antibody Names | Anti-Sperm-Associated Antigen 8 antibodyAnti-Hsd-1 antibodyAnti-Sperm Membrane Protein 1 antibodyAnti-Smp-1 antibodyAnti-Sperm Membrane Protein Bs-84 antibodyAnti-SPAG8 antibody |
Information sourced from Uniprot.org