| Host: |
Rabbit |
| Applications: |
IF |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SPAG5 (1-300) is suitable for use in Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
IF/ICC 1:20-1:50 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SPAG5 |
| Gene ID: |
10615 |
| Uniprot ID: |
SPAG5_HUMAN |
| Immunogen Region: |
1-300 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human SPAG5 (NP_006452.3). |
| Immunogen Sequence: |
MWRVKKLSLSLSPSPQTGKP SMRTPLRELTLQPGALTNSG KRSPACSSLTPSLCKLGLQE GSNNSSPVDFVNNKRTDLSS EHFSHSSKWLETCQHESDEQ PLDPIPQISSTPKTSEEAVD PLGNYMVKTIVLVPSPLGQQ QDMIFEARLDTMAETNSISL NGPLRTDDLVREEVAPCMGD RFSEVAAVSEKPIFQESPSH LLEESPPNPCSEQLHCSKES LSSRTEAVREDLVPSESNA |
| Tissue Specificity | Highly expressed in testis. Detected at low levels in placenta, liver, pancreas, thymus and colon. |
| Post Translational Modifications | Phosphorylated by AURKA. |
| Function | Essential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase. Required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture. In complex with SKAP, promotes stable microtubule-kinetochore attachments. May contribute to the regulation of separase activity. May regulate AURKA localization to mitotic spindle, but not to centrosomes and CCNB1 localization to both mitotic spindle and centrosomes. Involved in centriole duplication. Required for CDK5RAP2, CEP152, WDR62 and CEP63 centrosomal localization and promotes the centrosomal localization of CDK2. In non-mitotic cells, upon stress induction, inhibits mammalian target of rapamycin complex 1 (mTORC1) association and recruits the mTORC1 component RPTOR to stress granules (SGs), thereby preventing mTORC1 hyperactivation-induced apoptosis. May enhance GSK3B-mediated phosphorylation of other substrates, such as MAPT/TAU. |
| Protein Name | Sperm-Associated Antigen 5AstrinDeepestMitotic Spindle-Associated Protein P126Map126 |
| Cellular Localisation | CytoplasmCytoskeletonSpindleSpindle PoleChromosomeCentromereKinetochoreMidbodyMicrotubule Organizing CenterCentrosomeCytoplasmic GranuleCentriolar SatelliteColocalizes With Pcm1 At Centriolar Satellites Throughout The Cell CycleIn A Punctate Pattern In Interphase CellsDuring MitosisDetected At Spindle Poles During ProphaseThroughout The Spindle In Metaphase And AnaphaseAnd At Midzone Microtubules In Anaphase And TelophaseEfficient Targeting To The Mitotic Spindle May Depend Upon Phosphorylation By Gsk3bDetected On Kinetochores Of Chromosomes That Have CongressedThe Astrin (Spag5)-Kinastrin (Skap) Complex Localizes To The Microtubule Plus EndsIn Non-Mitotic Non-Stressed CellsShows A Microtubuli PatternIn Arsenite-Stressed CellsAccumulates In Stress Granules |
| Alternative Antibody Names | Anti-Sperm-Associated Antigen 5 antibodyAnti-Astrin antibodyAnti-Deepest antibodyAnti-Mitotic Spindle-Associated Protein P126 antibodyAnti-Map126 antibodyAnti-SPAG5 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89098/357557/STJ11100026_1__69013.1713121681.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
