Anti-SPAG4 antibody (STJA0008124)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human SPAG4
General Information
| Short Description | Rabbit polyclonal anti-Sperm-associated antigen 4 protein for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SPAG4 |
| Gene ID | 6676 |
| Uniprot ID | SPAG4_HUMAN |
| Immunogen | Synthesized peptide derived from human SPAG4 |
| Specificity | This antibody detects endogenous levels of SPAG4 at Human, Mouse, Rat |
Additional Info
| Function | Involved in spermatogenesis. Required for sperm head formation but not required to establish and maintain general polarity of the sperm head. Required for anchoring and organization of the manchette. Required for targeting of SUN3 and probably SYNE1 through a probable SUN1:SYNE3 LINC complex to the nuclear envelope and involved in accurate posterior sperm head localization of the complex. May anchor SUN3 the nuclear envelope. Involved in maintenance of the nuclear envelope integrity. May assist the organization and assembly of outer dense fibers (ODFs), a specific structure of the sperm tail. |
| Protein Name | Sperm-Associated Antigen 4 ProteinOuter Dense Fiber-Associated Protein Spag4Sun Domain-Containing Protein 4 |
| Database Links | |
| Cellular Localisation | MembraneMulti-Pass Membrane ProteinCytoplasmCytoskeletonFlagellum AxonemeNucleus EnvelopeNucleus Inner MembraneIn SpermatidsIt Is Localized In The Transient Manchette And In The Axoneme Of Elongating Spermatids And Epididymal SpermColocalized With Sept12 At The Nuclear Periphery In Round SpermatidsAt Sperm Neck In Elongated Spermatids And At Midpiece Regions In Ejaculated Spermatozoa |
| Alternative Antibody Names | Anti-Sperm-Associated Antigen 4 Protein antibodyAnti-Outer Dense Fiber-Associated Protein Spag4 antibodyAnti-Sun Domain-Containing Protein 4 antibodyAnti-SPAG4 antibodyAnti-SUN4 antibody |
Information sourced from Uniprot.org