• STJ72138 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ72138 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-SPAG16 antibody (139-152) (STJ72138)

SKU:
STJ72138-100

£303.50
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Host: Goat
Applications: Pep-ELISA/FC/IF/IHC
Reactivity: Human/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-SPAG16 (139-152) is suitable for use in ELISA, Flow Cytometry, Immunofluorescence and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IF-Strong expression of the protein seen in the cytoplasm of A431 cells. 10µg/ml
FC-Flow cytometric analysis of A431 cells. 10ug/ml
ELISA-antibody detection limit dilution 1:16000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: SPAG16
Gene ID: 79582
Uniprot ID: SPG16_HUMAN
Immunogen Region: 139-152
Accession Number: NP_078808.3; NP_001020607.1
Specificity: This antibody is expected to recognize isoform 1 and 2 (NP_078808.3; NP_001020607.1).
Immunogen Sequence: KGVTELRTVGNVPD
Post Translational Modifications Phosphorylated by TSSK2.
Function Necessary for sperm flagellar function. Plays a role in motile ciliogenesis. May help to recruit STK36 to the cilium or apical surface of the cell to initiate subsequent steps of construction of the central pair apparatus of motile cilia.
Protein Name Sperm-Associated Antigen 16 Protein
Pf20 Protein Homolog
Cellular Localisation Cytoplasm
Cytoskeleton
Flagellum Axoneme
Cilium Axoneme
Cell Projection
Cilium
Flagellum
Detected On The Sperm Flagellum Axoneme
Detected In The Central Apparatus Of The Axoneme
Colocalizes With Spag6 On Microtubules
Alternative Antibody Names Anti-Sperm-Associated Antigen 16 Protein antibody
Anti-Pf20 Protein Homolog antibody
Anti-SPAG16 antibody
Anti-PF20 antibody

Information sourced from Uniprot.org

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