Anti-SPAG16 antibody (139-152) (STJ72138)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-SPAG16 (139-152) is suitable for use in ELISA, Flow Cytometry, Immunofluorescence and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/FC/IF/IHC |
| Host | Goat |
| Reactivity | Human/Dog/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:16000.IHC: This antibody was successfully used in Human and Murine frozen Brain sections (PMID: 25086173).IF: Strong expression of the protein seen in the cytoplasm of A431 cells. Recommend |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | SPAG16 |
| Gene ID | 79582 |
| Uniprot ID | SPG16_HUMAN |
| Accession Number | NP_078808.3; NP_001020607.1 |
| Immunogen Region | 139-152 |
| Immunogen Sequence | KGVTELRTVGNVPD |
| Specificity | This antibody is expected to recognize isoform 1 and 2 (NP_078808.3; NP_001020607.1). |
Additional Info
| Post Translational Modifications | Phosphorylated by TSSK2. |
| Function | Necessary for sperm flagellar function. Plays a role in motile ciliogenesis. May help to recruit STK36 to the cilium or apical surface of the cell to initiate subsequent steps of construction of the central pair apparatus of motile cilia. |
| Protein Name | Sperm-Associated Antigen 16 ProteinPf20 Protein Homolog |
| Cellular Localisation | CytoplasmCytoskeletonFlagellum AxonemeCilium AxonemeCell ProjectionCiliumFlagellumDetected On The Sperm Flagellum AxonemeDetected In The Central Apparatus Of The AxonemeColocalizes With Spag6 On Microtubules |
| Alternative Antibody Names | Anti-Sperm-Associated Antigen 16 Protein antibodyAnti-Pf20 Protein Homolog antibodyAnti-SPAG16 antibodyAnti-PF20 antibody |
Information sourced from Uniprot.org