Anti-Sp1 (mouse)-Mouse antibody (Internal) (STJ73318)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-Sp1 (mouse)-Mouse (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Mouse/Rat/Pig |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 100kDa band observed in nuclear lysates of cell line NIH3T3 (calculated MW of 80.4kDa according to NP_038700.2). This molecular weight is routinely observed by other sources. Rec |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Accession Number | NP_038700.2 |
| Immunogen Region | Internal |
| Immunogen Sequence | DGIHDETAGGEGEN |
Additional Info
| Function | Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter Plays an essential role in the regulation of FE65 gene expression. Positively regulates the transcription of the core clock component ARNTL/BMAL1. Plays a role in protecting cells against oxidative stress following brain injury by regulating the expression of RNF112. |
| Protein Name | Transcription Factor Sp1Specificity Protein 1 |
| Database Links | Reactome: R-MMU-2173796Reactome: -MMU-6807505Reactome: -MMU-9018519 |
| Cellular Localisation | NucleusCytoplasmNuclear Location Is Governed By Glycosylated/Phosphorylated StatesInsulin Promotes Nuclear LocationWhile Glucagon Favors Cytoplasmic Location |
| Alternative Antibody Names | Anti-Transcription Factor Sp1 antibodyAnti-Specificity Protein 1 antibodyAnti-Sp1 antibody |
Information sourced from Uniprot.org