Anti-Sortilin antibody [R02-6B4] (STJA0035365)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenRecombinant protein of human Sortilin/NT3
General Information
| Short Description | Rabbit monoclonal anti-Sortilin for use in WB and IHC-P in Human and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R02-6B4 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100 |
| Formulation | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | SORT1 |
| Gene ID | 6272 |
| Uniprot ID | SORT_HUMAN |
| Immunogen | Recombinant protein of human Sortilin/NT3 |
Additional Info
| Tissue Specificity | Expressed in brain and prostate (at protein level). Expressed at high levels in brain, spinal cord, heart, skeletal muscle, thyroid, placenta and testis. Expressed at lower levels in lymphoid organs, kidney, colon and liver. |
| Post Translational Modifications | The N-terminal propeptide is cleaved by furin and possibly other homologous proteases. Palmitoylated. Undergoes cysteine S-palmitoylation which promotes the partitioning of the receptor into an endosomal membrane subdomain where it can interact with the retromer cargo-selective complex which mediates its retrograde trafficking to the Golgi apparatus. Phosphorylation at Ser-825 facilitates the interaction with GGA1. |
| Function | Functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). Lysosomal proteins bind specifically to the receptor in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelysosomal compartment where the low pH mediates the dissociation of the complex. The receptor is then recycled back to the Golgi for another round of trafficking through its binding to the retromer. Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles, or GSVs). These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin. May also mediate transport from the endoplasmic reticulum to the Golgi. |
| Protein Name | Sortilin100 Kda Nt ReceptorGlycoprotein 95Gp95Neurotensin Receptor 3Nt3Ntr3 |
| Database Links | Reactome: R-HSA-432722 |
| Cellular Localisation | Golgi ApparatusGolgi Stack MembraneSingle-Pass Type I Membrane ProteinEndosome MembraneEndoplasmic Reticulum MembraneNucleus MembraneCell MembraneExtracellular SideLysosome MembraneLocalized To Membranes Of The Endoplasmic ReticulumEndosomesGolgi StackLysosomes And NucleusA Small Fraction Of The Protein Is Also Localized To The Plasma MembraneMay Also Be Found In Slc2a4/Glut4 Storage Vesicles (Gsvs) In AdipocytesLocalization To The Plasma Membrane In Adipocytes May Be Enhanced By Insulin |
| Alternative Antibody Names | Anti-Sortilin antibodyAnti-100 Kda Nt Receptor antibodyAnti-Glycoprotein 95 antibodyAnti-Gp95 antibodyAnti-Neurotensin Receptor 3 antibodyAnti-Nt3 antibodyAnti-Ntr3 antibodyAnti-SORT1 antibody |
Information sourced from Uniprot.org