Anti-Sortilin antibody (ECD) (STJ13100315)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from the extracellular domain of rat Sortilin conjugated to blue carrier protein was used as the antigen.
General Information
| Short Description | Rabbit polyclonal anti-Sortilin (ECD) for use in IHC and WB in Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB. A dilution of 1:1000 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | Sort1 |
| Gene ID | 20661 |
| Uniprot ID | SORT_MOUSE |
| Immunogen | A synthetic peptide from the extracellular domain of rat Sortilin conjugated to blue carrier protein was used as the antigen. |
| Immunogen Region | ECD |
| Specificity | Specific for Sortilin. |
Additional Info
| Post Translational Modifications | The N-terminal propeptide is cleaved by furin and possibly other homologous proteases. Phosphorylation at Ser-819 facilitates the interaction with GGA1. Palmitoylated. Undergoes cysteine S-palmitoylation which promotes the partitioning of the receptor into an endosomal membrane subdomain where it can interact with the retromer cargo-selective complex which mediates its retrograde trafficking to the Golgi apparatus. |
| Function | Functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). Lysosomal proteins bind specifically to the receptor in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelysosomal compartment where the low pH mediates the dissociation of the complex. The receptor is then recycled back to the Golgi for another round of trafficking through its binding to the retromer. Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles, or GSVs). These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin. May also mediate transport from the endoplasmic reticulum to the Golgi. |
| Protein Name | SortilinNeurotensin Receptor 3Ntr3Mntr3 |
| Database Links | Reactome: R-MMU-432722 |
| Cellular Localisation | Golgi ApparatusGolgi Stack MembraneSingle-Pass Type I Membrane ProteinEndosome MembraneEndoplasmic Reticulum MembraneNucleus MembraneCell MembraneExtracellular SideLysosome MembraneLocalized To Membranes Of The Endoplasmic ReticulumEndosomesGolgi StackLysosomes And NucleusA Small Fraction Of The Protein Is Also Localized To The Plasma MembraneMay Also Be Found In Slc2a4/Glut4 Storage Vesicles (Gsvs) In AdipocytesLocalization To The Plasma Membrane In Adipocytes May Be Enhanced By Insulin |
| Alternative Antibody Names | Anti-Sortilin antibodyAnti-Neurotensin Receptor 3 antibodyAnti-Ntr3 antibodyAnti-Mntr3 antibodyAnti-Sort1 antibody |
Information sourced from Uniprot.org