Anti-SORBS1/ponsin antibody (Internal) (STJ70890)

SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ70890-100
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General Information

Short DescriptionGoat polyclonal antibody anti-SORBS1/ponsin (Internal) is suitable for use in ELISA and Immunohistochemistry research applications.
ApplicationsPep-ELISA/IHC
HostGoat
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:2000.
WB: Preliminary experiments in Human Adipose, Heart, Kidney, Liver and Muscle lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml).
IHC: Paraffin
Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20°C on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolSORBS1
Gene ID10580
Uniprot IDSRBS1_HUMAN
Accession NumberNP_006425.2; NP_056200.1; NP_001030126.1; NP_001030127.1; NP_001030128.1; NP_079267.1; NP_001030129.1
Immunogen RegionInternal
Immunogen SequenceSPKSEDDDSDLYSP
SpecificityThis antibody is expected to recognise all 7 reported isoforms (NP_006425.2, NP_056200.1, NP_001030126.1, NP_001030127.1, NP_001030128.1, NP_079267.1 and NP_001030129.1).

Additional Info

Post Translational Modifications O-glycosylated.
Function Plays a role in tyrosine phosphorylation of CBL by linking CBL to the insulin receptor. Required for insulin-stimulated glucose transport. Involved in formation of actin stress fibers and focal adhesions.
Protein Name Sorbin And Sh3 Domain-Containing Protein 1
Ponsin
Sh3 Domain Protein 5
Sh3p12
C-Cbl-Associated Protein
Cap
Database Links Reactome: R-HSA-445355
Cellular Localisation Cell Junction
Adherens Junction
Cell Membrane
Cytoplasm
Cytoskeleton
Focal Adhesion
Nucleus
Nucleus Matrix
Colocalizes With The Ten-1 Icd Form Of Tenm1 In The Nucleus
Colocalizes With Actin Stress Fibers
Also Detected At The Plasma Membrane And In Neuronal Intranuclear Inclusions
Colocalized With Pxn At Focal Adhesions During Myogenic Differentiation
Alternative Antibody Names Anti-Sorbin And Sh3 Domain-Containing Protein 1 antibody
Anti-Ponsin antibody
Anti-Sh3 Domain Protein 5 antibody
Anti-Sh3p12 antibody
Anti-C-Cbl-Associated Protein antibody
Anti-Cap antibody
Anti-SORBS1 antibody
Anti-KIAA0894 antibody
Anti-KIAA1296 antibody
Anti-SH3D5 antibody

Information sourced from Uniprot.org

Citations

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