• Western blot analysis of extracts of various cell lines, using SOD3 antibody (STJ29064) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST.
  • Western blot analysis of extracts of various cell lines, using SOD3 rabbit polyclonal antibody (STJ29064) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded human mammary cancer using SOD3 antibody (STJ29064) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat ovary using SOD3 antibody (STJ29064) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse pancreas using SOD3 antibody (STJ29064) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-SOD3 antibody (19-160) (STJ29064)

SKU:
STJ29064

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SOD3 (19-160) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SOD3
Gene ID: 6649
Uniprot ID: SODE_HUMAN
Immunogen Region: 19-160
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 19-160 of human SOD3 (NP_003093.2).
Immunogen Sequence: WTGEDSAEPNSDSAEWIRDM YAKVTEIWQEVMQRRDDDGA LHAACQVQPSATLDAAQPRV TGVVLFRQLAPRAKLDAFFA LEGFPTEPNSSSRAIHVHQF GDLSQGCESTGPHYNPLAVP HPQHPGDFGNFAVRDGSLWR YR
Tissue Specificity Expressed in blood vessels, heart, lung, kidney and placenta. Major SOD isoenzyme in extracellular fluids such as plasma, lymph and synovial fluid.
Function Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.
Protein Name Extracellular Superoxide Dismutase Cu-Zn
Ec-Sod
Database Links Reactome: R-HSA-3299685
Cellular Localisation Secreted
Extracellular Space
Golgi Apparatus
Trans-Golgi Network
99% Of Ec-Sod Is Anchored To Heparan Sulfate Proteoglycans In The Tissue Interstitium
And 1% Is Located In The Vasculature In Equilibrium Between The Plasma And The Endothelium
Alternative Antibody Names Anti-Extracellular Superoxide Dismutase Cu-Zn antibody
Anti-Ec-Sod antibody
Anti-SOD3 antibody

Information sourced from Uniprot.org

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