• Western blot analysis of extracts of various cell lines, using SOCS5 antibody (STJ110261) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using SOCS5 antibody (STJ110261) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human mammary cancer using SOCS5 antibody (STJ110261) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using SOCS5 antibody (STJ110261) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human liver cancer using SOCS5 antibody (STJ110261) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human mammary cancer using SOCS5 antibody (STJ110261) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human lung cancer using SOCS5 antibody (STJ110261) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using SOCS5 antibody (STJ110261) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-SOCS5 antibody (1-140) (STJ110261)

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STJ110261

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SOCS5 (1-140) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SOCS5
Gene ID: 9655
Uniprot ID: SOCS5_HUMAN
Immunogen Region: 1-140
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human SOCS5 (NP_054730.1).
Immunogen Sequence: MDKVGKMWNNFKYRCQNLFG HEGGSRSENVDMNSNRCLSV KEKNISIGDSTPQQQSSPLR ENIALQLGLSPSKNSSRRNQ NCATEIPQIVEISIEKDNDS CVTPGTRLARRDSYSRHAPW GGKKKHSCSTKTQSSLDADK
Post Translational Modifications Phosphorylated. Phosphorylation is induced by EGF.
Function SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Inhibits for instance EGF signaling by mediating the degradation of the EGF receptor/EGFR. Involved in the regulation of T-helper cell differentiation by inhibiting of the IL4 signaling pathway which promotes differentiation into the Th2 phenotype. Can also partially inhibit IL6 and LIF signaling.
Protein Name Suppressor Of Cytokine Signaling 5
Socs-5
Cytokine-Inducible Sh2 Protein 6
Cis-6
Cytokine-Inducible Sh2-Containing Protein 5
Database Links Reactome: R-HSA-6785807
Reactome: R-HSA-8951664
Alternative Antibody Names Anti-Suppressor Of Cytokine Signaling 5 antibody
Anti-Socs-5 antibody
Anti-Cytokine-Inducible Sh2 Protein 6 antibody
Anti-Cis-6 antibody
Anti-Cytokine-Inducible Sh2-Containing Protein 5 antibody
Anti-SOCS5 antibody
Anti-CIS6 antibody
Anti-CISH5 antibody
Anti-CISH6 antibody
Anti-KIAA0671 antibody

Information sourced from Uniprot.org

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