Anti-SOAT2 antibody (STJA0008280)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human SOAT2
General Information
| Short Description | Rabbit polyclonal anti-Sterol O-acyltransferase 2 for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SOAT2 |
| Gene ID | 8435 |
| Uniprot ID | SOAT2_HUMAN |
| Immunogen | Synthesized peptide derived from human SOAT2 |
| Specificity | This antibody detects endogenous levels of SOAT2 at Human, Mouse, Rat |
Additional Info
| Post Translational Modifications | Polyubiquitinated by AMFR/gp78 at Cys-277, leading to its degradation when the lipid levels are low. Association with AMFR/gp78 is mediated via interaction with INSIG1. High concentration of cholesterol and fatty acid results in Cys-277 oxidation, preventing ubiquitination at the same site, resulting in protein stabilization. Oxidized at Cys-277: high concentration of cholesterol and fatty acid induce reactive oxygen species, which oxidizes Cys-277, preventing ubiquitination at the same site, and resulting in protein stabilization. |
| Function | Catalyzes the formation of fatty acid-cholesterol esters, which are less soluble in membranes than cholesterol. Plays a role in lipoprotein assembly and dietary cholesterol absorption. Utilizes oleoyl-CoA ((9Z)-octadecenoyl-CoA) and linolenoyl-CoA ((9Z,12Z,15Z)-octadecatrienoyl-CoA) as substrates. May provide cholesteryl esters for lipoprotein secretion from hepatocytes and intestinal mucosa. Isoform 2: Has lower enzymatic activity compared to isoform 1. Isoform 3: Has lower enzymatic activity compared to isoform 1. |
| Protein Name | Sterol O-Acyltransferase 2Acyl-Coenzyme A -Cholesterol Acyltransferase 2Acat-2Cholesterol Acyltransferase 2 |
| Database Links | Reactome: R-HSA-8964038 |
| Cellular Localisation | Endoplasmic Reticulum MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Sterol O-Acyltransferase 2 antibodyAnti-Acyl-Coenzyme A -Cholesterol Acyltransferase 2 antibodyAnti-Acat-2 antibodyAnti-Cholesterol Acyltransferase 2 antibodyAnti-SOAT2 antibodyAnti-ACACT2 antibodyAnti-ACAT2 antibody |
Information sourced from Uniprot.org