• Immunofluorescence analysis of C6 cells using SOAT1 Rabbit polyclonal antibody (STJ28233) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using SOAT1 Rabbit polyclonal antibody (STJ28233) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using SOAT1 Rabbit polyclonal antibody (STJ28233) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using SOAT1 antibody (STJ28233) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SOAT1 antibody (1-130) (STJ28233)

SKU:
STJ28233

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SOAT1 (1-130) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SOAT1
Gene ID: 6646
Uniprot ID: SOAT1_HUMAN
Immunogen Region: 1-130
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human SOAT1 (NP_003092.4).
Immunogen Sequence: MVGEEKMSLRNRLSKSRENP EEDEDQRNPAKESLETPSNG RIDIKQLIAKKIKLTAEAEE LKPFFMKEVGSHFDDFVTNL IEKSASLDNGGCALTTFSVL EGEKNNHRAKDLRAPPEQGK IFIARRSLLD
Function Catalyzes the formation of fatty acid-cholesterol esters, which are less soluble in membranes than cholesterol. Plays a role in lipoprotein assembly and dietary cholesterol absorption. Utilizes oleoyl-CoA ((9Z)-octadecenoyl-CoA) preferentially as susbstrate: shows a higher activity towards an acyl-CoA substrate with a double bond at the delta-9 position (9Z) than towards saturated acyl-CoA or an unsaturated acyl-CoA with a double bond at the delta-7 (7Z) or delta-11 (11Z) positions.
Protein Name Sterol O-Acyltransferase 1
Acyl-Coenzyme A -Cholesterol Acyltransferase 1
Acat-1
Cholesterol Acyltransferase 1
Database Links Reactome: R-HSA-8964038
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Sterol O-Acyltransferase 1 antibody
Anti-Acyl-Coenzyme A -Cholesterol Acyltransferase 1 antibody
Anti-Acat-1 antibody
Anti-Cholesterol Acyltransferase 1 antibody
Anti-SOAT1 antibody
Anti-ACACT antibody
Anti-ACACT1 antibody
Anti-ACAT antibody
Anti-ACAT1 antibody
Anti-SOAT antibody
Anti-STAT antibody

Information sourced from Uniprot.org

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