• Western blot analysis of extracts of various cell lines, using SNX3 antibody (STJ29638) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SNX3 antibody (1-162) (STJ29638)

SKU:
STJ29638

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SNX3 (1-162) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SNX3
Gene ID: 8724
Uniprot ID: SNX3_HUMAN
Immunogen Region: 1-162
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-162 of human SNX3 (NP_003786.1).
Immunogen Sequence: MAETVADTRRLITKPQNLND AYGPPSNFLEIDVSNPQTVG VGRGRFTTYEIRVKTNLPIF KLKESTVRRRYSDFEWLRSE LERESKVVVPPLPGKAFLRQ LPFRGDDGIFDDNFIEERKQ GLEQFINKVAGHPLAQNERC LHMFLQDEIIDKSYTPSKIR HA
Post Translational Modifications Ubiquitinated, leading to its proteasomal degradation. Deubiquitinated by USP10.
Function Phosphoinositide-binding protein required for multivesicular body formation. Specifically binds phosphatidylinositol 3-phosphate (PtdIns(P3)). Can also bind phosphatidylinositol 4-phosphate (PtdIns(P4)), phosphatidylinositol 5-phosphate (PtdIns(P5)) and phosphatidylinositol 3,5-biphosphate (PtdIns(3,5)P2). Plays a role in protein transport between cellular compartments. Together with RAB7A facilitates endosome membrane association of the retromer cargo-selective subcomplex (CSC/VPS). May in part act as component of the SNX3-retromer complex which mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway. Promotes stability and cell surface expression of epithelial sodium channel (ENAC) subunits SCNN1A and SCNN1G. Not involved in EGFR degradation. Involved in the regulation of phagocytosis in dendritic cells possibly by regulating EEA1 recruitment to the nascent phagosomes. Involved in iron homeostasis through regulation of endocytic recycling of the transferrin receptor TFRC presumably by delivering the transferrin:transferrin receptor complex to recycling endosomes.the function may involve the CSC retromer subcomplex. In the case of Salmonella enterica infection plays arole in maturation of the Salmonella-containing vacuole (SCV) and promotes recruitment of LAMP1 to SCVs.
Protein Name Sorting Nexin-3
Protein Sdp3
Database Links Reactome: R-HSA-3238698
Reactome: R-HSA-5689880
Cellular Localisation Early Endosome
Cytoplasmic Vesicle
Phagosome
Colocalizes To Clathrin-Coated Endosomal Vesicles Morphologically Distinct From Retromer-Decorated Non-Branched Endosomal Tubule Structures Colocalizes With Eea1 On Nascent Phagosomes In Dendritic Cells But Competes With Eea1 For Binding To Phagosomal Membrane
In The Case Of Salmonella Enterica Infection Localizes To Salmonella-Containing Vacuoles (Scvs) From Which Snx3-Containing Tubules Form 30-60 Min After Infection
Alternative Antibody Names Anti-Sorting Nexin-3 antibody
Anti-Protein Sdp3 antibody
Anti-SNX3 antibody

Information sourced from Uniprot.org

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