• Immunohistochemistry analysis of SNRPF in paraffin-embedded rat heart using SNRPF Rabbit polyclonal antibody (STJ114055) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of SNRPF in paraffin-embedded mouse testis using SNRPF Rabbit polyclonal antibody (STJ114055) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of SNRPF in paraffin-embedded human colon carcinoma using SNRPF Rabbit polyclonal antibody (STJ114055) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using SNRPF Rabbit polyclonal antibody (STJ114055) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SNRPF antibody (1-86) (STJ114055)

SKU:
STJ114055

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SNRPF (1-86) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SNRPF
Gene ID: 6636
Uniprot ID: RUXF_HUMAN
Immunogen Region: 1-86
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-86 of human SNRPF (NP_003086.1).
Immunogen Sequence: MSLPLNPKPFLNGLTGKPVM VKLKWGMEYKGYLVSVDGYM NMQLANTEEYIDGALSGHLG EVLIRCNNVLYIRGVEEEEE DGEMRE
Function Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Component of both the pre-catalytic spliceosome B complex and activated spliceosome C complexes. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs. As part of the U7 snRNP it is involved in histone 3'-end processing.
Protein Name Small Nuclear Ribonucleoprotein F
Snrnp-F
Sm Protein F
Sm-F
Smf
Database Links Reactome: R-HSA-111367
Reactome: R-HSA-191859
Reactome: R-HSA-72163
Reactome: R-HSA-72165
Reactome: R-HSA-73856
Reactome: R-HSA-77588
Reactome: R-HSA-9754678
Cellular Localisation Cytoplasm
Cytosol
Nucleus
Smn-Mediated Assembly Into Core Snrnps Occurs In The Cytosol Before Smn-Mediated Transport To The Nucleus To Be Included In Spliceosomes
Alternative Antibody Names Anti-Small Nuclear Ribonucleoprotein F antibody
Anti-Snrnp-F antibody
Anti-Sm Protein F antibody
Anti-Sm-F antibody
Anti-Smf antibody
Anti-SNRPF antibody
Anti-PBSCF antibody

Information sourced from Uniprot.org

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