![Western blot analysis of lysates from wild type (WT) and SNRNP70 knockdown (KD) 293T cells using [KD Validated] SNRNP70 Rabbit monoclonal antibody (STJ11105291) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177935/369955/STJ11105291_1__41750.1713135864.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and SNRNP70 knockdown (KD) 293T cells using [KD Validated] SNRNP70 Rabbit monoclonal antibody (STJ11105291) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit Exposure time: 90s.")
![Western blot analysis of lysates from Mouse thymus using [KD Validated] SNRNP70 Rabbit monoclonal antibody (STJ11105291) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177935/369956/STJ11105291_2__32490.1713135865.jpg?c=1 "Western blot analysis of lysates from Mouse thymus using [KD Validated] SNRNP70 Rabbit monoclonal antibody (STJ11105291) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western blot analysis of lysates from wild type (WT) and SNRNP70 knockdown (KD) 293T cells using [KD Validated] SNRNP70 Rabbit monoclonal antibody (STJ11105291) at 1:3000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177935/369957/STJ11105291_3__95433.1713135868.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and SNRNP70 knockdown (KD) 293T cells using [KD Validated] SNRNP70 Rabbit monoclonal antibody (STJ11105291) at 1:3000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 90s.")
![Western blot analysis of lysates from wild type (WT) and SNRNP70 knockdown (KD) 293T cells using [KD Validated] SNRNP70 Rabbit monoclonal antibody (STJ11105291) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/177935/369955/STJ11105291_1__41750.1713135864.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and SNRNP70 knockdown (KD) 293T cells using [KD Validated] SNRNP70 Rabbit monoclonal antibody (STJ11105291) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit Exposure time: 90s.")
Anti-SNRNP70 antibody (201-300) [S5291RM] (STJ11105291)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal U1 Small Nuclear Ribonucleoprotein 70 Kda (201-300) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S5291RM |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:5000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SNRNP70 |
| Gene ID | 6625 |
| Uniprot ID | RU17_HUMAN |
| Immunogen Region | 201-300 |
| Immunogen Sequence | RGGADVNIRHSGRDDTSRYD ERPGPSPLPHRDRDRDRERE RRERSRERDKERERRRSRSR DRRRRSRSRDKEERRRSRER SKDKDRDRKRRSSRSRERAR |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 201-300 of SNRNP70 (NP_003080.2). |
Additional Info
| Post Translational Modifications | The N-terminus is blocked. Extensively phosphorylated on serine residues in the C-terminal region. |
| Function | Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. SNRNP70 binds to the loop I region of U1-snRNA. Isoform 3: Truncated isoforms that lack the RRM domain cannot bind U1-snRNA. Isoform 4: Truncated isoforms that lack the RRM domain cannot bind U1-snRNA. |
| Protein Name | U1 Small Nuclear Ribonucleoprotein 70 KdaU1 Snrnp 70 KdaU1-70kSnrnp70 |
| Database Links | Reactome: R-HSA-72163 |
| Cellular Localisation | Nucleus SpeckleNucleusNucleoplasmColocalizes With Scnm1 And Luc7l2 In Nuclear Speckles |
| Alternative Antibody Names | Anti-U1 Small Nuclear Ribonucleoprotein 70 Kda antibodyAnti-U1 Snrnp 70 Kda antibodyAnti-U1-70k antibodyAnti-Snrnp70 antibodyAnti-SNRNP70 antibodyAnti-RNPU1Z antibodyAnti-RPU1 antibodyAnti-SNRP70 antibodyAnti-U1AP1 antibody |
Information sourced from Uniprot.org