| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SNRNP70 |
| Gene ID: | 6625 |
| Uniprot ID: | RU17_HUMAN |
| Immunogen Region: | 1-180 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human SNRNP70 (NP_003080.2). |
| Immunogen Sequence: | MTQFLPPNLLALFAPRDPIP YLPPLEKLPHEKHHNQPYCG IAPYIREFEDPRDAPPPTRA ETREERMERKRREKIERRQQ EVETELKMWDPHNDPNAQGD AFKTLFVARVNYDTTESKLR REFEVYGPIKRIHMVYSKRS GKPRGYAFIEYEHERDMHSA YKHADGKKIDGRRVLVDVER |
| Post Translational Modifications | The N-terminus is blocked. Extensively phosphorylated on serine residues in the C-terminal region. |
| Function | Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. SNRNP70 binds to the loop I region of U1-snRNA. Isoform 3: Truncated isoforms that lack the RRM domain cannot bind U1-snRNA. Isoform 4: Truncated isoforms that lack the RRM domain cannot bind U1-snRNA. |
| Protein Name | U1 Small Nuclear Ribonucleoprotein 70 KdaU1 Snrnp 70 KdaU1-70kSnrnp70 |
| Database Links | Reactome: R-HSA-72163 |
| Cellular Localisation | Nucleus SpeckleNucleusNucleoplasmColocalizes With Scnm1 And Luc7l2 In Nuclear Speckles |
| Alternative Antibody Names | Anti-U1 Small Nuclear Ribonucleoprotein 70 Kda antibodyAnti-U1 Snrnp 70 Kda antibodyAnti-U1-70k antibodyAnti-Snrnp70 antibodyAnti-SNRNP70 antibodyAnti-RNPU1Z antibodyAnti-RPU1 antibodyAnti-SNRP70 antibodyAnti-U1AP1 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and SNRNP70 knockdown (KD) 293T cells using [KD Validated] SNRNP70 Rabbit monoclonal antibody (STJ11105291) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/177935/369955/STJ11105291_1__41750.1713135864.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and SNRNP70 knockdown (KD) 293T cells using [KD Validated] SNRNP70 Rabbit monoclonal antibody (STJ11105291) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit Exposure time: 90s.")
