Anti-SNCA antibody (62-112) {Biotin} (STJ501862)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationBiotin
IsotypeIgG
ImmunogenSynthetic peptide taken within amino acid region 62-112Β unique sequence on alpha-synuclein isoform NACP 112Β protein.
General Information
| Short Description | Rabbit polyclonal antibody anti-SNCA (62-112) is suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and Western Blot research applications. |
| Applications | ELISA/IHC/IP/WB |
| Host | Rabbit |
| Reactivity | Human/Monkey/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Biotin |
| Concentration | 0.68-0.70 Β΅g/Β΅l |
| Purification | Affinity Purified |
| Dilution Range | WB: 1:500DB: 1:20, 000ELISA: 1:20, 000IP: 1:200IHC: 1:100 |
| Formulation | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Storage Instruction | Store at-20Β°C for long term storage. Avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | SNCA |
| Gene ID | 6622 |
| Uniprot ID | SYUA_HUMAN |
| Immunogen | Synthetic peptide taken within amino acid region 62-112Β unique sequence on alpha-synuclein isoform NACP 112Β protein. |
| Immunogen Region | 62-112 |
| Specificity | This antibody will not detect NACP140. |
Additional Info
| Tissue Specificity | Highly expressed in presynaptic terminals in the central nervous system. Expressed principally in brain. |
| Post Translational Modifications | Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress. Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers. Ubiquitinated. The predominant conjugate is the diubiquitinated form. Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure. |
| Function | Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis. Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. This chaperone activity is important to sustain normal SNARE-complex assembly during aging. Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity. |
| Protein Name | Alpha-SynucleinNon-A Beta Component Of Ad AmyloidNon-A4 Component Of Amyloid PrecursorNacp |
| Database Links | Reactome: R-HSA-977225Reactome: R-HSA-9833482 |
| Cellular Localisation | CytoplasmMembraneNucleusSynapseSecretedCell ProjectionAxonMembrane-Bound In Dopaminergic NeuronsExpressed And Colocalized With Septin4 In Dopaminergic Axon TerminalsEspecially At The Varicosities |
| Alternative Antibody Names | Anti-Alpha-Synuclein antibodyAnti-Non-A Beta Component Of Ad Amyloid antibodyAnti-Non-A4 Component Of Amyloid Precursor antibodyAnti-Nacp antibodyAnti-SNCA antibodyAnti-NACP antibodyAnti-PARK1 antibody |
Information sourced from Uniprot.org