Anti-SNAI2 antibody (152-202) (STJA0010130)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human SLUG AA range: 152-202
General Information
| Short Description | Rabbit polyclonal anti-Zinc finger protein SNAI2 (152-202) for use in WB, ELISA and IHC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA/IHC |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000IHC-p 1:50-300ELISA 2000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SNAI2 |
| Gene ID | 6591 |
| Uniprot ID | SNAI2_HUMAN |
| Immunogen | Synthesized peptide derived from human SLUG AA range: 152-202 |
| Immunogen Region | 152-202 |
| Specificity | This antibody detects endogenous levels of Human, Mouse, Rat SLUG |
Additional Info
| Tissue Specificity | Expressed in most adult human tissues, including spleen, thymus, prostate, testis, ovary, small intestine, colon, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Not detected in peripheral blood leukocyte. Expressed in the dermis and in all layers of the epidermis, with high levels of expression in the basal layers (at protein level). Expressed in osteoblasts (at protein level). Expressed in mesenchymal stem cells (at protein level). Expressed in breast tumor cells (at protein level). |
| Post Translational Modifications | Phosphorylated by GSK3B. Once phosphorylated, it becomes a target for ubiquitination. Ubiquitinated by the SCF(FBXO11) complex.ubiquitination requires previous GSK3B-mediated SNAI2 phosphorylation. |
| Function | Transcriptional repressor that modulates both activator-dependent and basal transcription. Involved in the generation and migration of neural crest cells. Plays a role in mediating RAF1-induced transcriptional repression of the TJ protein, occludin (OCLN) and subsequent oncogenic transformation of epithelial cells. Represses BRCA2 expression by binding to its E2-box-containing silencer and recruiting CTBP1 and HDAC1 in breast cells. In epidermal keratinocytes, binds to the E-box in ITGA3 promoter and represses its transcription. Involved in the regulation of ITGB1 and ITGB4 expression and cell adhesion and proliferation in epidermal keratinocytes. Binds to E-box2 domain of BSG and activates its expression during TGFB1-induced epithelial-mesenchymal transition (EMT) in hepatocytes. Represses E-Cadherin/CDH1 transcription via E-box elements. Involved in osteoblast maturation. Binds to RUNX2 and SOC9 promoters and may act as a positive and negative transcription regulator, respectively, in osteoblasts. Binds to CXCL12 promoter via E-box regions in mesenchymal stem cells and osteoblasts. Plays an essential role in TWIST1-induced EMT and its ability to promote invasion and metastasis. |
| Protein Name | Zinc Finger Protein Snai2Neural Crest Transcription Factor SlugProtein Snail Homolog 2 |
| Database Links | Reactome: R-HSA-8943724Reactome: R-HSA-9856649 |
| Cellular Localisation | NucleusCytoplasmObserved In Discrete Foci In Interphase NucleiThese Nuclear Foci Do Not Overlap With The NucleoliThe Sp100 And The Hp1 Heterochromatin Or The Coiled BodySuggesting Snai2 Is Associated With Active Transcription Or Active Splicing Regions |
| Alternative Antibody Names | Anti-Zinc Finger Protein Snai2 antibodyAnti-Neural Crest Transcription Factor Slug antibodyAnti-Protein Snail Homolog 2 antibodyAnti-SNAI2 antibodyAnti-SLUG antibodyAnti-SLUGH antibody |
Information sourced from Uniprot.org