• Immunofluorescence analysis of MCF-7 cells using SMYD1 antibody (STJ26205). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse heart using SMYD1 antibody (STJ26205) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung using SMYD1 antibody (STJ26205) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using SMYD1 antibody (STJ26205) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using SMYD1 Antibody (STJ26205) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-SMYD1 antibody (1-350) (STJ26205)

SKU:
STJ26205

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SMYD1 (1-350) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IHC-P 1:20-1:200
IF/ICC 1:20-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SMYD1
Gene ID: 150572
Uniprot ID: SMYD1_HUMAN
Immunogen Region: 1-350
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human SMYD1 (NP_938015.1).
Immunogen Sequence: MTIGRMENVEVFTAEGKGRG LKATKEFWAADIIFAERAYS AVVFDSLVNFVCHTCFKRQE KLHRCGQCKFAHYCDRTCQK DAWLNHKNECSAIKRYGKVP NENIRLAARIMWRVEREGTG LTEGCLVSVDDLQNHVEHFG EEEQKDLRVDVDTFLQYWPP QSQQFSMQYISHIFGVINCN GFTLSDQRGLQAVGVGIFPN LGLVNHDCWPNCTVIFNNGN HEAVKSMFHTQMRIELRAL
Tissue Specificity Expression seems mostly restricted to heart and skeletal muscle.
Function Methylates histone H3 at 'Lys-4' (H3K4me), seems able to perform both mono-, di-, and trimethylation. Acts as a transcriptional repressor. Essential for cardiomyocyte differentiation and cardiac morphogenesis.
Protein Name Histone-Lysine N-Methyltransferase Smyd1
Set And Mynd Domain-Containing Protein 1
Database Links Reactome: R-HSA-9733709
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Histone-Lysine N-Methyltransferase Smyd1 antibody
Anti-Set And Mynd Domain-Containing Protein 1 antibody
Anti-SMYD1 antibody

Information sourced from Uniprot.org

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