| Short Description : | Rabbit polyclonal SMUG1 (1-270) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:200-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SMUG1 |
| Gene ID: | 23583 |
| Uniprot ID: | SMUG1_HUMAN |
| Immunogen Region: | 1-270 |
| Immunogen Sequence: | MPQAFLLGSIHEPAGALMEP QPCPGSLAESFLEEELRLNA ELSQLQFSEPVGIIYNPVEY AWEPHRNYVTRYCQGPKEVL FLGMNPGPFGMAQTGVPFGE VSMVRDWLGIVGPVLTPPQE HPKRPVLGLECPQSEVSGAR FWGFFRNLCGQPEVFFHHCF VHNLCPLLFLAPSGRNLTPA ELPAKQREQLLGICDAALCR QVQLLGVRLVVGVGRLAEQR ARRALAGLMPEVQVEGLLH |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human SMUG1 (NP_055126.1). |
| Function | Recognizes base lesions in the genome and initiates base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA with a preference for single-stranded DNA substrates. The activity is greater toward mismatches (U/G) compared to matches (U/A). Excises uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 5-hydroxyuracil (hoU) and 5-hydroxymethyluracil (hmU) in ssDNA and dsDNA, but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine), nor other oxidized bases. The activity is damage-specific and salt-dependent. The substrate preference is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at low salt concentration, and dsDNA (G pair) > dsDNA (A pair) > ssDNA at high salt concentration. |
| Protein Name | Single-Strand Selective Monofunctional Uracil Dna Glycosylase |
| Database Links | Reactome: R-HSA-110328Reactome: R-HSA-110329Reactome: R-HSA-110357 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Single-Strand Selective Monofunctional Uracil Dna Glycosylase antibodyAnti-SMUG1 antibody |
Information sourced from Uniprot.org
