• Western blot analysis of extracts of various cell lines, using SMTN antibody (STJ28828) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 5min.

Anti-SMTN antibody (65-325) (STJ28828)

SKU:
STJ28828

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SMTN
Gene ID: 6525
Uniprot ID: SMTN_HUMAN
Immunogen Region: 65-325
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 65-325 of human SMTN (NP_001193946.1).
Immunogen Sequence: EGALRKLLEVTADLAERRRI RSAIRELQRQELEREEEALA SKRFRAERQDNKENWLHSQQ REAEQRAALARLAGQLESMN DVEELTALLRSAGEYEERKL IRAAIRRVRAQEIEAATLAG RLYSGRPNSGSREDSKGLAA HRLEQCEVPEREEQEQQAEV SKPTPTPEGTSQDVTTVTLL LRAPPGSTSSSPASPSSSPT PASPEPPLEPAEAQCLTAEV PGSPEPPPSPPKTTSPEPQ
Tissue Specificity Smooth muscle.contractile or vascular (for the long form).
Function Structural protein of the cytoskeleton.
Protein Name Smoothelin
Cellular Localisation Cytoplasm
Cytoskeleton
Exhibits A Filamentous Organization
Alternative Antibody Names Anti-Smoothelin antibody
Anti-SMTN antibody
Anti-SMSMO antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance