Anti-SMO/Smoothened antibody [SAA1978] (STJA0020406)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2a
General Information
| Short Description | Mouse monoclonal Protein Smoothened antibody for use in ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO) MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SAA1978 |
| Isotype | IgG2a |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Protein A/G purified from cell culture supernatant |
| Formulation | 0.01M PBS, pH 7.4. |
| Storage Instruction | Suitable for storage at +4°C between 1-2 weeks For longer term store at-20°C for up to 12 months |
Target Information
| Gene Symbol | SMO |
| Gene ID | 6608 |
| Uniprot ID | SMO_HUMAN |
Additional Info
| Function | G protein-coupled receptor which associates with the patched protein (PTCH) to transduce hedgehog protein signaling. Binding of sonic hedgehog (SHH) to its receptor patched prevents inhibition of smoothened (SMO) by patched. When active, SMO binds to and sequesters protein kinase A catalytic subunit PRKACA at the cell membrane, preventing PRKACA-mediated phosphorylation of GLI transcription factors which releases the GLI proteins from PRKACA-mediated inhibition and allows for transcriptional activation of hedgehog pathway target genes. Required for the accumulation of KIF7, GLI2 and GLI3 in the cilia. Interacts with DLG5 at the ciliary base to induce the accumulation of KIF7 and GLI2 at the ciliary tip for GLI2 activation. |
| Protein Name | Protein SmoothenedProtein Gx |
| Database Links | Reactome: R-HSA-373080Reactome: R-HSA-5610787Reactome: R-HSA-5620922Reactome: R-HSA-5632684Reactome: R-HSA-5635838 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinCell ProjectionCiliumCilium Localization Is Promoted By Shh And Is Required For Activity |
| Alternative Antibody Names | Anti-Protein Smoothened antibodyAnti-Protein Gx antibodyAnti-SMO antibodyAnti-SMOH antibody |
Information sourced from Uniprot.org