Anti-SMN antibody [4B7] (STJA0003856)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG
ImmunogenRecombinant human his-tagged SMN protein expressed in and purified from E. coli. The epitope recognized by the 4B7 clone binds to the region encoded by exon 1.
General Information
| Short Description | Mouse monoclonal anti-Survival Motor Neuron for use in WB and ICC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ICC |
| Host | Mouse |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 4B7 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | This antibody was protein g purified culture from supernatant. |
| Dilution Range | WB 1:4000ICC 1:50-1:500 |
| Formulation | 100 ยตl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 ยตg per ml BSA and 50% Glycerol. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SMN1.SMN2 |
| Gene ID | 6606/6607 |
| Uniprot ID | SMN_HUMAN |
| Immunogen | Recombinant human his-tagged SMN protein expressed in and purified from E. coli. The epitope recognized by the 4B7 clone binds to the region encoded by exon 1. |
Additional Info
| Tissue Specificity | Expressed in a wide variety of tissues. Expressed at high levels in brain, kidney and liver, moderate levels in skeletal and cardiac muscle, and low levels in fibroblasts and lymphocytes. Also seen at high levels in spinal cord. Present in osteoclasts and mononuclear cells (at protein level). |
| Function | The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A forming an intermediate. Within the SMN complex, SMN1 acts as a structural backbone and together with GEMIN2 it gathers the Sm complex subunits. Binding of snRNA inside 5Sm ultimately triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP. Ensures the correct splicing of U12 intron-containing genes that may be important for normal motor and proprioceptive neurons development. Also required for resolving RNA-DNA hybrids created by RNA polymerase II, that form R-loop in transcription terminal regions, an important step in proper transcription termination. May also play a role in the metabolism of small nucleolar ribonucleoprotein (snoRNPs). |
| Protein Name | Survival Motor Neuron ProteinComponent Of Gems 1Gemin-1 |
| Database Links | Reactome: R-HSA-191859Reactome: R-HSA-9754678 |
| Cellular Localisation | NucleusGemCajal BodyCytoplasmCytoplasmic GranulePerikaryonCell ProjectionNeuron ProjectionAxonMyofibrilSarcomereZ LineColocalizes With Actin And At The Z-Line Of Skeletal MuscleUnder Stress Conditions Colocalizes With Rpp20/Pop7 In Punctuated Cytoplasmic GranulesColocalized And Redistributed With Zpr1 From The Cytoplasm To Nuclear Gems (Gemini Of Coiled Bodies) And Cajal BodiesColocalizes With Fmr1 In Cytoplasmic Granules In The Soma And Neurite Cell Processes |
| Alternative Antibody Names | Anti-Survival Motor Neuron Protein antibodyAnti-Component Of Gems 1 antibodyAnti-Gemin-1 antibodyAnti-SMN1 antibodyAnti-SMN antibodyAnti-SMNT.SMN2 antibodyAnti-SMNC antibody |
Information sourced from Uniprot.org