| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SMG7 (1-300) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:200-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SMG7 |
| Gene ID: |
9887 |
| Uniprot ID: |
SMG7_HUMAN |
| Immunogen Region: |
1-300 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human SMG7 (NP_963863.2). |
| Immunogen Sequence: |
MSLQSAQYLRQAEVLKADMT DSKLGPAEVWTSRQALQDLY QKMLVTDLEYALDKKVEQDL WNHAFKNQITTLQGQAKNRA NPNRSEVQANLSLFLEAASG FYTQLLQELCTVFNVDLPCR VKSSQLGIISNKQTHTSAIV KPQSSSCSYICQHCLVHLGD IARYRNQTSQAESYYRHAAQ LVPSNGQPYNQLAILASSKG DHLTTIFYYCRSIAVKFPFP AASTNLQKALSKALESRDE |
| Function | Plays a role in nonsense-mediated mRNA decay. Recruits UPF1 to cytoplasmic mRNA decay bodies. Together with SMG5 is thought to provide a link to the mRNA degradation machinery involving exonucleolytic pathways, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation. |
| Protein Name | Nonsense-Mediated Mrna Decay Factor Smg7Smg-7 HomologHsmg-7 |
| Database Links | Reactome: R-HSA-975957 |
| Cellular Localisation | CytoplasmNucleusPredominantly CytoplasmicAnd NuclearShuttles Between Nucleus And Cytoplasm |
| Alternative Antibody Names | Anti-Nonsense-Mediated Mrna Decay Factor Smg7 antibodyAnti-Smg-7 Homolog antibodyAnti-Hsmg-7 antibodyAnti-SMG7 antibodyAnti-C1orf16 antibodyAnti-EST1C antibodyAnti-KIAA0250 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89098/357557/STJ11100026_1__69013.1713121681.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
