Anti-SMC3 antibody [18B6] (STJA0032588)
SPECIFICATIONS
ClonalityMonoclonal
HostRat
ConjugationUnconjugated
IsotypeIgG2a
ImmunogenRecombinant mouse SMC3
General Information
| Short Description | Rat monoclonal anti-SMC3 for use in WB in Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rat |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 18B6 |
| Isotype | IgG2a |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000 |
| Formulation | Liquid in PBS containing 0.09% sodium azide as a preservative. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | Smc3 |
| Gene ID | 13006 |
| Uniprot ID | SMC3_MOUSE |
| Immunogen | Recombinant mouse SMC3 |
Additional Info
| Tissue Specificity | Spermatocytes (at protein level). Widely expressed, with higher expression in testis and brain. |
| Post Translational Modifications | Phosphorylated at Ser-1083 in a SPO11-dependent manner. Acetylation at Lys-105 and Lys-106 by ESCO1 is important for genome stability and S phase sister chromatid cohesion. Regulated by DSCC1, it is required for processive DNA synthesis, coupling sister chromatid cohesion establishment during S phase to DNA replication. Deacetylation by HDAC8, regulates release of the cohesin complex from chromatin. Ubiquitinated by the DCX(DCAF15) complex, leading to its degradation. |
| Function | Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex also plays an important role in spindle pole assembly during mitosis and in chromosomes movement. |
| Protein Name | Structural Maintenance Of Chromosomes Protein 3Smc Protein 3Smc-3Basement Membrane-Associated Chondroitin ProteoglycanBamacanChondroitin Sulfate Proteoglycan 6Chromosome Segregation Protein SmcdMad Member-Interacting Protein 1 |
| Database Links | Reactome: R-MMU-2467813Reactome: -MMU-2468052Reactome: -MMU-2470946Reactome: -MMU-2500257Reactome: -MMU-3108214 |
| Cellular Localisation | NucleusChromosomeCentromereAssociates With ChromatinBefore Prophase It Is Scattered Along Chromosome ArmsDuring ProphaseMost Of Cohesin Complexes Dissociate From Chromatin Probably Because Of Phosphorylation By PlkExcept At CentromeresWhere Cohesin Complexes RemainAt AnaphaseThe Rad21 Subunit Of The Cohesin Complex Is CleavedLeading To The Dissociation Of The Complex From ChromosomesAllowing Chromosome SeparationThe Phosphorylated Form At Ser-1083 Is Preferentially Associated With Unsynapsed Chromosomal Regions |
| Alternative Antibody Names | Anti-Structural Maintenance Of Chromosomes Protein 3 antibodyAnti-Smc Protein 3 antibodyAnti-Smc-3 antibodyAnti-Basement Membrane-Associated Chondroitin Proteoglycan antibodyAnti-Bamacan antibodyAnti-Chondroitin Sulfate Proteoglycan 6 antibodyAnti-Chromosome Segregation Protein Smcd antibodyAnti-Mad Member-Interacting Protein 1 antibodyAnti-Smc3 antibodyAnti-Bam antibodyAnti-Bmh antibodyAnti-Cspg6 antibodyAnti-Mmip1 antibodyAnti-Smc3l1 antibodyAnti-Smcd antibody |
Information sourced from Uniprot.org