• Western blot analysis of extracts of various cell lines, using Smarcd1 antibody (STJ111991) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using Smarcd1 antibody (STJ111991) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat brain using Smarcd1 antibody (STJ111991) (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using Smarcd1 antibody (STJ111991) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human lung cancer using Smarcd1 antibody (STJ111991) (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded rat brain using Smarcd1 antibody (STJ111991) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse brain using Smarcd1 antibody (STJ111991) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using Smarcd1 antibody (STJ111991) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-SMARCD1 antibody (1-120) (STJ111991)

SKU:
STJ111991

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BAF60a (1-120) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SMARCD1
Gene ID: 6602
Uniprot ID: SMRD1_HUMAN
Immunogen Region: 1-120
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of mouse BAF60a (NP_114030.2).
Immunogen Sequence: MAARAGFQSVAPSGGAGASG GAGVAAALGPGGTPGPPVRM GPAPGQGLYRSPMPGAAYPR PGMLPGSRMTPQGPSMGPPG YGGNPSVRPGLAQSGMDQSR KRPAPQQIQQVQQQAVQNRN
Tissue Specificity Expressed in all tissues tested, including brain, heart, kidney, liver, lung, muscle, pancreas and placenta.
Function Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. Has a strong influence on vitamin D-mediated transcriptional activity from an enhancer vitamin D receptor element (VDRE). May be a link between mammalian SWI-SNF-like chromatin remodeling complexes and the vitamin D receptor (VDR) heterodimer. Mediates critical interactions between nuclear receptors and the BRG1/SMARCA4 chromatin-remodeling complex for transactivation. Interacts with AKIRIN2.
Protein Name Swi/Snf-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily D Member 1
60 Kda Brg-1/Brm-Associated Factor Subunit A
Brg1-Associated Factor 60a
Baf60a
Swi/Snf Complex 60 Kda Subunit
Database Links Reactome: R-HSA-3214858
Reactome: R-HSA-8939243
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Swi/Snf-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily D Member 1 antibody
Anti-60 Kda Brg-1/Brm-Associated Factor Subunit A antibody
Anti-Brg1-Associated Factor 60a antibody
Anti-Baf60a antibody
Anti-Swi/Snf Complex 60 Kda Subunit antibody
Anti-SMARCD1 antibody
Anti-BAF60A antibody

Information sourced from Uniprot.org

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