• Western blot analysis of lysate from K562 cells, using Brg-1 antibody.

Anti-SMARCA4 antibody (1565-1614 aa) (STJ91889)

SKU:
STJ91889

Current Stock:
Host: Rabbit
Applications: WB/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Transcription activator BRG1 (1565-1614 aa) is suitable for use in Western Blot, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IF ICC 1:100-500
ELISA 1:5000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SMARCA4
Gene ID: 6597
Uniprot ID: SMCA4_HUMAN
Immunogen Region: 1565-1614 aa
Specificity: Brg-1 Polyclonal Antibody detects endogenous levels of Brg-1 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Brg-1 at the amino acid range 1565-1614
Function Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating the calcium-dependent release of a repressor complex and the recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by SMARCA4-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves the release of HDAC1 and recruitment of CREBBP. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development, a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. SMARCA4/BAF190A may promote neural stem cell self-renewal/proliferation by enhancing Notch-dependent proliferative signals, while concurrently making the neural stem cell insensitive to SHH-dependent differentiating cues. Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1. Binds via DLX1 to enhancers located in the intergenic region between DLX5 and DLX6 and this binding is stabilized by the long non-coding RNA (lncRNA) Evf2. Binds to RNA in a promiscuous manner. Binding to RNAs including lncRNA Evf2 leads to inhibition of SMARCA4 ATPase and chromatin remodeling activities. In brown adipose tissue, involved in the regulation of thermogenic genes expression.
Protein Name Transcription Activator Brg1
Atp-Dependent Helicase Smarca4
Brg1-Associated Factor 190a
Baf190a
Mitotic Growth And Transcription Activator
Protein Brg-1
Protein Brahma Homolog 1
Snf2-Beta
Swi/Snf-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily A Member 4
Database Links Reactome: R-HSA-1266695
Reactome: R-HSA-201722
Reactome: R-HSA-3214858
Reactome: R-HSA-3247509
Reactome: R-HSA-8939243
Reactome: R-HSA-9619665
Cellular Localisation Nucleus
Colocalizes With Long Non-Coding Rna Evf2 In Nuclear Rna Clouds
Localizes To Sites Of Dna Damage
Alternative Antibody Names Anti-Transcription Activator Brg1 antibody
Anti-Atp-Dependent Helicase Smarca4 antibody
Anti-Brg1-Associated Factor 190a antibody
Anti-Baf190a antibody
Anti-Mitotic Growth And Transcription Activator antibody
Anti-Protein Brg-1 antibody
Anti-Protein Brahma Homolog 1 antibody
Anti-Snf2-Beta antibody
Anti-Swi/Snf-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily A Member 4 antibody
Anti-SMARCA4 antibody
Anti-BAF190A antibody
Anti-BRG1 antibody
Anti-SNF2B antibody
Anti-SNF2L4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance