| Host: |
Rabbit |
| Applications: |
IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SMAD9 (1-300) is suitable for use in Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SMAD9 |
| Gene ID: |
4093 |
| Uniprot ID: |
SMAD9_HUMAN |
| Immunogen Region: |
1-300 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human Smad9 (NP_005896.1). |
| Immunogen Sequence: |
MHSTTPISSLFSFTSPAVKR LLGWKQGDEEEKWAEKAVDS LVKKLKKKKGAMDELERALS CPGQPSKCVTIPRSLDGRLQ VSHRKGLPHVIYCRVWRWPD LQSHHELKPLECCEFPFGSK QKEVCINPYHYRRVETPVLP PVLVPRHSEYNPQLSLLAKF RSASLHSEPLMPHNATYPDS FQQPPCSALPPSPSHAFSQS PCTASYPHSPGSPSEPESPY QHSDFRPVCYEEPQHWCSV |
| Tissue Specificity | Expressed in heart, brain, placenta, lung, skeletal muscle, prostate, testis, ovary and small intestine. Also expressed in fetal brain, lung and kidney. |
| Post Translational Modifications | Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase. |
| Function | Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD9 is a receptor-regulated SMAD (R-SMAD). |
| Protein Name | Mothers Against Decapentaplegic Homolog 9Mad Homolog 9Mothers Against Dpp Homolog 9Madh6Smad Family Member 9Smad 9Smad9 |
| Database Links | Reactome: R-HSA-201451 |
| Cellular Localisation | CytoplasmNucleusIn The Cytoplasm In The Absence Of LigandMigration To The Nucleus When Complexed With Smad4 |
| Alternative Antibody Names | Anti-Mothers Against Decapentaplegic Homolog 9 antibodyAnti-Mad Homolog 9 antibodyAnti-Mothers Against Dpp Homolog 9 antibodyAnti-Madh6 antibodyAnti-Smad Family Member 9 antibodyAnti-Smad 9 antibodyAnti-Smad9 antibodyAnti-SMAD9 antibodyAnti-MADH6 antibodyAnti-MADH9 antibodyAnti-SMAD8 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89098/357557/STJ11100026_1__69013.1713121681.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
