• Immunofluorescence analysis of HeLa cells using Smad5 monoclonal antibody.
  • Flow cytometric analysis of Jurkat cells stained with Smad5 monoclonal antibody (red) , followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control, normal mouse IgG.
  • Western blot analysis using Smad5 monoclonal antibody against mouse testis lysate.

Anti-SMAD5 antibody (C-Term) (STJ98549)

SKU:
STJ98549

£139.00 - £336.00
Current Stock:
Host: Mouse
Applications: WB/IF/FC
Reactivity: Human/Mouse/Rat/Cow/Chicken/Pig/Sheep
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Mothers against decapentaplegic homolog 5 (C-Term) is suitable for use in Western Blot, Immunofluorescence and Flow Cytometry research applications.
Clonality: Monoclonal
Conjugation: Unconjugated
Formulation: Liquid in 0.1M Tris-Glycine (pH7.4, 150 mM NaCl) , 0.2% Sodium Azide and 50% Glycerol.
Purification: Affinity purification
Concentration: 1 mg/mL
Dilution Range: WB 1:1000-1:2000
IF 1:100-1:500
FC 1:100-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SMAD5
Gene ID: 4090
Uniprot ID: SMAD5_HUMAN
Immunogen Region: C-Term
Specificity: Smad5 Monoclonal Antibody detects endogenous levels of Smad5 protein.
Immunogen: Purified recombinant human Smad5 (C-terminus) protein fragments expressed in E.coli.
Post Translational Modifications Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase. Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
Function Transcriptional regulator that plays a role in various cellular processes including embryonic development, cell differentiation, angiogenesis and tissue homeostasis. Upon BMP ligand binding to their receptors at the cell surface, is phosphorylated by activated type I BMP receptors (BMPRIs) and associates with SMAD4 to form an heteromeric complex which translocates into the nucleus acting as transcription factor. In turn, the hetero-trimeric complex recognizes cis-regulatory elements containing Smad Binding Elements (SBEs) to modulate the outcome of the signaling network. Non-phosphorylated SMAD5 has a cytoplasmic role in energy metabolism regulation by promoting mitochondrial respiration and glycolysis in response to cytoplasmic pH changes. Mechanistically, interacts with hexokinase 1/HK1 and thereby accelerates glycolysis.
Protein Name Mothers Against Decapentaplegic Homolog 5
Mad Homolog 5
Mothers Against Dpp Homolog 5
Jv5-1
Smad Family Member 5
Smad 5
Smad5
Hsmad5
Database Links Reactome: R-HSA-201451
Cellular Localisation Cytoplasm
Nucleus
Mitochondrion
Cytoplasmic In The Absence Of Ligand
Migrates To The Nucleus When Complexed With Smad4
Alternative Antibody Names Anti-Mothers Against Decapentaplegic Homolog 5 antibody
Anti-Mad Homolog 5 antibody
Anti-Mothers Against Dpp Homolog 5 antibody
Anti-Jv5-1 antibody
Anti-Smad Family Member 5 antibody
Anti-Smad 5 antibody
Anti-Smad5 antibody
Anti-Hsmad5 antibody
Anti-SMAD5 antibody
Anti-MADH5 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance