Anti-SMAC/Diablo antibody (56-239aa) [AT19F2] (STJA0041661)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2ak
ImmunogenRecombinant human SMAC/Diablo (56-239aa) purified from E. coli
General Information
| Short Description | Mouse monoclonal anti-SMAC/Diablo (56-239aa) for use in ELISA, WB, ICC, IF and FACS in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA/WB/ICC/IF/FACS |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | AT19F2 |
| Isotype | IgG2ak |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | By protein-A affinity chromatography |
| Formulation | Liquid in phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DIABLO |
| Gene ID | 56616 |
| Uniprot ID | DBLOH_HUMAN |
| Accession Number | NP_063940 |
| Immunogen | Recombinant human SMAC/Diablo (56-239aa) purified from E. coli |
| Immunogen Region | 56-239aa |
Additional Info
| Tissue Specificity | Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed. |
| Post Translational Modifications | Ubiquitinated by BIRC7/livin. Ubiquitinated by BIRC6. The precursor form is proteolytically cleaved by mitochondrial processing peptidase MPP to remove the transit peptide and produce an intermediate form. This is then processed by PARL to produce the mature cleaved form which is released from mitochondria into the cytosol in apoptotic cells. |
| Function | Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/BRUCE by inhibiting its binding to caspases. Isoform 3: Attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1: Defective in the capacity to down-regulate the XIAP/BIRC4 abundance. |
| Protein Name | Diablo Iap-Binding Mitochondrial ProteinDiablo Homolog - MitochondrialDirect Iap-Binding Protein With Low PiSecond Mitochondria-Derived Activator Of CaspasesSmac Cleaved Into - Diablo Iap-Binding Mitochondrial Protein - Cleaved Form |
| Database Links | Reactome: R-HSA-111457Reactome: R-HSA-111463Reactome: R-HSA-111464Reactome: R-HSA-111469Reactome: R-HSA-9627069 |
| Cellular Localisation | MitochondrionCytoplasmCytosolReleased Into The Cytosol In A Parl-Dependent Manner When Cells Undergo Apoptosis |
| Alternative Antibody Names | Anti-Diablo Iap-Binding Mitochondrial Protein antibodyAnti-Diablo Homolog - Mitochondrial antibodyAnti-Direct Iap-Binding Protein With Low Pi antibodyAnti-Second Mitochondria-Derived Activator Of Caspases antibodyAnti-Smac Cleaved Into - Diablo Iap-Binding Mitochondrial Protein - Cleaved Form antibodyAnti-DIABLO antibodyAnti-SMAC antibody |
Information sourced from Uniprot.org