Anti-SLIT2 antibody (1400-1500) [S7MR] (STJ11101877)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal Slit2 (1400-1500) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S7MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:2000IHC-P:1:200-1:800ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SLIT2 |
| Gene ID | 9353 |
| Uniprot ID | SLIT2_HUMAN |
| Immunogen Region | 1400-1500 |
| Immunogen Sequence | GHGGVLCDEEEDLFNPCQAI KCKHGKCRLSGLGQPYCECS SGYTGDSCDREISCRGERIR DYYQKQQGYAACQTTKKVSR LECRGGCAGGQCCGPLRSKR R |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1400-1500 of human Slit2 (O94813). |
Additional Info
| Tissue Specificity | Fetal lung and kidney, and adult spinal cord. Weak expression in adult adrenal gland, thyroid, trachea and other tissues examined. |
| Function | Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal cord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal cord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration. |
| Protein Name | Slit Homolog 2 ProteinSlit-2 Cleaved Into - Slit Homolog 2 Protein N-Product - Slit Homolog 2 Protein C-Product |
| Database Links | Reactome: R-HSA-373752Reactome: R-HSA-376176Reactome: R-HSA-428540Reactome: R-HSA-428542Reactome: R-HSA-428543Reactome: R-HSA-428890Reactome: R-HSA-8985586Reactome: R-HSA-9010553Reactome: R-HSA-9830674 |
| Cellular Localisation | SecretedThe C-Terminal Cleavage Protein Is More Diffusible Than The Larger N-Terminal Protein That Is More Tightly Cell Associated |
| Alternative Antibody Names | Anti-Slit Homolog 2 Protein antibodyAnti-Slit-2 Cleaved Into - Slit Homolog 2 Protein N-Product - Slit Homolog 2 Protein C-Product antibodyAnti-SLIT2 antibodyAnti-SLIL3 antibody |
Information sourced from Uniprot.org