• Western blot analysis of various lysates using SLCO1B1 Rabbit polyclonal antibody (STJ11102844) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SLCO1B1 antibody (597-691) (STJ11102844)

SKU:
STJ11102844

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SLCO1B1 (597-691) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLCO1B1
Gene ID: 10599
Uniprot ID: SO1B1_HUMAN
Immunogen Region: 597-691
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 597-691 of human SLCO1B1 (NP_006437.3).
Immunogen Sequence: TTCIKWSTNNCGTRGSCRTY NSTSFSRVYLGLSSMLRVSS LVLYIILIYAMKKKYQEKDI NASENGSVMDEANLESLNKN KHFVPSAGADSETHC
Tissue Specificity Highly expressed in liver, at the basolateral membranes of centrilobular hepatocytes. Expressed in liver (at protein level). Expressed in fetal liver. Not detected in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocyte. In testis, primarily localized to the basal membrane of Sertoli cells and weakly expressed in Leydig cells and within the tubules.
Function Mediates the Na(+)-independent uptake of organic anions. Shows broad substrate specificity, can transport both organic anions such as bile acid taurocholate (cholyltaurine) and conjugated steroids (dehydroepiandrosterone 3-sulfate, 17-beta-glucuronosyl estradiol, and estrone 3-sulfate), as well as eicosanoids (prostaglandin E2, thromboxane B2, leukotriene C4, and leukotriene E4), and thyroid hormones (T4/L-thyroxine, and T3/3,3',5'-triiodo-L-thyronine). Can take up bilirubin glucuronides from plasma into the liver, contributing to the detoxification-enhancing liver-blood shuttling loop. Involved in the clearance of endogenous and exogenous substrates from the liver. Transports coproporphyrin I and III, by-products of heme synthesis, and may be involved in their hepatic disposition. May contribute to regulate the transport of organic compounds in testes across the blood-testis-barrier (Probable). Can transport HMG-CoA reductase inhibitors (also known as statins), such as pravastatin and pitavastatin, a clinically important class of hypolipidemic drugs. May play an important role in plasma and tissue distribution of the structurally diverse chemotherapeutic drug methotrexate. May also transport antihypertension agents, such as the angiotensin-converting enzyme (ACE) inhibitor prodrug enalapril, and the highly selective angiotensin II AT1-receptor antagonist valsartan, in the liver. Shows a pH-sensitive substrate specificity towards prostaglandin E2 and T4 which may be ascribed to the protonation state of the binding site and leads to a stimulation of substrate transport in an acidic microenvironment. Hydrogencarbonate/HCO3(-) acts as the probable counteranion that exchanges for organic anions.
Protein Name Solute Carrier Organic Anion Transporter Family Member 1b1
Slco1b1
Liver-Specific Organic Anion Transporter 1
Lst-1
Oatp-C
Organic Anion Transporter Slc21a6
Sodium-Independent Organic Anion-Transporting Polypeptide 2
Oatp-2
Solute Carrier Family 21 Member 6
Database Links Reactome: R-HSA-159418
Reactome: R-HSA-189483
Reactome: R-HSA-5619110
Reactome: R-HSA-879518
Reactome: R-HSA-9754706
Cellular Localisation Basolateral Cell Membrane
Multi-Pass Membrane Protein
Basal Cell Membrane
Detected In Basolateral Membranes Of Hepatocytes
Localized To The Basal Membrane Of Sertoli Cells
Alternative Antibody Names Anti-Solute Carrier Organic Anion Transporter Family Member 1b1 antibody
Anti-Slco1b1 antibody
Anti-Liver-Specific Organic Anion Transporter 1 antibody
Anti-Lst-1 antibody
Anti-Oatp-C antibody
Anti-Organic Anion Transporter Slc21a6 antibody
Anti-Sodium-Independent Organic Anion-Transporting Polypeptide 2 antibody
Anti-Oatp-2 antibody
Anti-Solute Carrier Family 21 Member 6 antibody
Anti-SLCO1B1 antibody
Anti-LST1 antibody
Anti-OATP1B1 antibody
Anti-OATP2 antibody
Anti-OATPC antibody
Anti-SLC21A6 antibody

Information sourced from Uniprot.org

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