• STJ71029 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing vesicle staining. The nuclear stain is DAPI (blue). NA NA NA Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ71029 Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing vesicle/ER staining. The nuclear stain is DAPI (blue). NA NA NA Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ71029 (0. 1µg/ml) staining of Human Smooth Muscle (A) and Tonsil (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence. This data is from a previous batch, not on sale.
  • STJ71029 (0. 3µg/ml) staining of A549 (A) and U2OS (B) cell ysate (35µg protein in RIPA buffer). Detected by chemiluminescence. This data is from a previous batch, not on sale.
  • STJ71029 Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. This data is from a previous batch, not on sale.

Anti-SLC7A11 antibody (Internal) (STJ71029)

SKU:
STJ71029-100

£303.50
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Current Stock:
Host: Goat
Applications: Pep-ELISA/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-SLC7A11 (Internal) is suitable for use in ELISA and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IF-Strong expression of the protein seen in vesicles of HepG2 cells and in vesicles/ER of A549 cells. 10µg/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: SLC7A11
Gene ID: 23657
Uniprot ID: XCT_HUMAN
Immunogen Region: Internal
Accession Number: NP_055146.1
Immunogen Sequence: KGQTQNFKDAFSGRD
Post Translational Modifications Ubiquitinated by TRIM26.leading to proteasomal degradation.
Function Heterodimer with SLC3A2, that functions as an antiporter by mediating the exchange of extracellular anionic L-cystine and intracellular L-glutamate across the cellular plasma membrane. Provides L-cystine for the maintenance of the redox balance between extracellular L-cystine and L-cysteine and for the maintenance of the intracellular levels of glutathione that is essential for cells protection from oxidative stress. The transport is sodium-independent, electroneutral with a stoichiometry of 1:1, and is drove by the high intracellular concentration of L-glutamate and the intracellular reduction of L-cystine. In addition, mediates the import of L-kynurenine leading to anti-ferroptotic signaling propagation required to maintain L-cystine and glutathione homeostasis. Moreover, mediates N-acetyl-L-cysteine uptake into the placenta leading to subsequently down-regulation of pathways associated with oxidative stress, inflammation and apoptosis. In vitro can also transport L-aspartate. May participate in astrocyte and meningeal cell proliferation during development and can provide neuroprotection by promoting glutathione synthesis and delivery from non-neuronal cells such as astrocytes and meningeal cells to immature neurons. Controls the production of pheomelanin pigment directly.
Protein Name Cystine/Glutamate Transporter
Amino Acid Transport System Xc-
Calcium Channel Blocker Resistance Protein Ccbr1
Solute Carrier Family 7 Member 11
Xct
Database Links Reactome: R-HSA-210991
Reactome: R-HSA-352230
Reactome: R-HSA-9818027
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Cell Projection
Microvillus Membrane
Localized To The Microvillous Membrane Of The Placental Syncytiotrophoblast
Alternative Antibody Names Anti-Cystine/Glutamate Transporter antibody
Anti-Amino Acid Transport System Xc- antibody
Anti-Calcium Channel Blocker Resistance Protein Ccbr1 antibody
Anti-Solute Carrier Family 7 Member 11 antibody
Anti-Xct antibody
Anti-SLC7A11 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance